BMRBj - BMREntryMaster

Found 1 Document (0.719 seconds)

BMRB: 15277

2JQF

Full Length Leader Protease of Foot and Mouth Disease Virus C51A Mutant

Authors

Cencic, R., Mayer, C., Juliano, M.A., Juliano, L., Konrat, R., Kontaxis, G., Skern, T.

Assembly

Leader Protease

Entity

1. Leader Protease (polymer, Thiol state: all free), 173 monomers, 19760.04 Da Detail

MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAKVQR KLK

Formula weight

19760.04 Da

Source organism

Foot-and-mouth disease virus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.7 %, Completeness: 33.9 %, Completeness (bb): 59.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.9 % (696 of 2056)	20.9 % (222 of 1060)	41.5 % (338 of 814)	74.7 % (136 of 182)
Backbone	59.1 % (600 of 1016)	44.8 % (155 of 346)	61.0 % (310 of 508)	83.3 % (135 of 162)
Sidechain	14.0 % (168 of 1202)	9.7 % (69 of 714)	20.9 % (98 of 468)	5.0 % (1 of 20)
Aromatic	6.2 % (15 of 242)	7.4 % (9 of 121)	5.2 % (6 of 115)	0.0 % (0 of 6)
Methyl	21.9 % (42 of 192)	18.8 % (18 of 96)	25.0 % (24 of 96)

1. Lbpro

MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAKVQR KLK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Leader Protease	[U-100% 13C; U-100% 15N]	1 mM
2	DTT	natural abundance	5 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	50 mM
5	sodium azide	natural abundance	10 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	Leader Protease	[U-100% 15N]	1 mM
9	DTT	natural abundance	5 mM
10	sodium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	50 mM
12	sodium azide	natural abundance	10 mM
13	H2O	natural abundance	90 %
14	D2O		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
15	Leader Protease	[U-100% 15N]	1 mM
16	DTT	natural abundance	5 mM
17	sodium phosphate	natural abundance	20 mM
18	sodium chloride	natural abundance	50 mM
19	sodium azide	natural abundance	10 mM
20	Pf1 phage	natural abundance	17 mg/mL
21	H2O	natural abundance	90 %
22	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.6 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JQF, Strand ID: R, S Detail

Release date

2007-07-04

Citation

Investigating the substrate specificity and oligomerisation of the leader protease of foot and mouth disease virus using NMR
Cencic, R., Mayer, C., Juliano, M.A., Juliano, L., Konrat, R., Kontaxis, G., Skern, T.
J. Mol. Biol. (2007), 373, 1071-1087, PubMed 17897674 , DOI 10.1016/j.jmb.2007.08.061 , Abstract

Related entities 1. Leader Protease, : 1 : 1 : 4 : 9 entities Detail

Interaction partners 1. Leader Protease, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC