Leader Protease
MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 46.4 % (914 of 1968) | 25.5 % (258 of 1011) | 64.5 % (504 of 782) | 86.9 % (152 of 175) |
Backbone | 81.7 % (801 of 980) | 52.7 % (176 of 334) | 96.7 % (474 of 490) | 96.8 % (151 of 156) |
Sidechain | 23.2 % (265 of 1144) | 12.3 % (83 of 677) | 40.4 % (181 of 448) | 5.3 % (1 of 19) |
Aromatic | 3.3 % (8 of 242) | 3.3 % (4 of 121) | 3.5 % (4 of 115) | 0.0 % (0 of 6) |
Methyl | 23.9 % (44 of 184) | 19.6 % (18 of 92) | 28.3 % (26 of 92) |
1. sLbpro
MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sLbpro | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | DTT | natural abundance | 5 mM | |
3 | sodium azide | natural abundance | 10 mM | |
4 | sodium phosphate | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 50 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | sLbpro | [U-100% 15N] | 1 mM | |
9 | DTT | natural abundance | 5 mM | |
10 | sodium azide | natural abundance | 10 mM | |
11 | sodium phosphate | natural abundance | 20 mM | |
12 | sodium chloride | natural abundance | 50 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | sLbpro | [U-100% 15N] | 1 mM | |
16 | DTT | natural abundance | 5 mM | |
17 | sodium azide | natural abundance | 10 mM | |
18 | sodium phosphate | natural abundance | 20 mM | |
19 | sodium chloride | natural abundance | 50 mM | |
20 | Pf1 phage | natural abundance | 17 mg/mL | |
21 | H2O | natural abundance | 90 % | |
22 | D2O | 10 % |