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BMRB: 15279

2JQ6

Structure of the EH-domain of EHD1

Authors

KIEKEN, F.P., JOVIC, M., CAPLAN, S., SORGEN, P.L.

Assembly

EH domain of EHD1 in presence of Ca+2

Entity

1. EH domain of EHD1 (polymer, Thiol state: not present), 139 monomers, 14971.80 Da Detail

GPLGSESLMP SQVVKGGAFD GTMNGPFGHG YGEGAGEGID DVEWVVGKDK PTYDEIFYTL SPVNGKITGA NAKKEMVKSK LPNTVLGKIW KLADVDKDGL LDDEEFALAN HLIKVKLEGH ELPADLPPHL VPPSKRRHE

2. CA (non-polymer), 40.078 Da

Total weight

15011.878 Da

Max. entity weight

14971.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 97.0 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.0 % (1534 of 1581)	97.2 % (801 of 824)	96.8 % (601 of 621)	97.1 % (132 of 136)
Backbone	96.8 % (786 of 812)	96.8 % (276 of 285)	96.5 % (385 of 399)	97.7 % (125 of 128)
Sidechain	97.4 % (867 of 890)	97.4 % (525 of 539)	97.7 % (335 of 343)	87.5 % (7 of 8)
Aromatic	95.4 % (103 of 108)	98.1 % (53 of 54)	94.2 % (49 of 52)	50.0 % (1 of 2)
Methyl	97.9 % (143 of 146)	97.3 % (71 of 73)	98.6 % (72 of 73)

1. EH domain of EHD1

GPLGSESLMP SQVVKGGAFD GTMNGPFGHG YGEGAGEGID DVEWVVGKDK PTYDEIFYTL SPVNGKITGA NAKKEMVKSK LPNTVLGKIW KLADVDKDGL LDDEEFALAN HLIKVKLEGH ELPADLPPHL VPPSKRRHE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	EH Domain of EHD-1	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	CALCIUM ION	natural abundance	3 mM
3	PBS	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.778 ppm	internal	indirect	0.2514495
¹H	water	protons	4.778 ppm	internal	direct	1.0
¹⁵N	water	protons	4.778 ppm	internal	indirect	0.1013291

Referencing offset: 1.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.778 ppm	internal	indirect	0.2514495
¹H	water	protons	4.778 ppm	internal	direct	1.0
¹⁵N	water	protons	4.778 ppm	internal	indirect	0.1013291

Referencing offset: 1.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.778 ppm	internal	indirect	0.2514495
¹H	water	protons	4.778 ppm	internal	direct	1.0
¹⁵N	water	protons	4.778 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.778 ppm	internal	indirect	0.2514495
¹H	water	protons	4.778 ppm	internal	direct	1.0
¹⁵N	water	protons	4.778 ppm	internal	indirect	0.1013291

Referencing offset: 1.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JQ6, Strand ID: A Detail

Release date

2007-10-15

Citation

EH domain of EHD1
Kieken, F.P., Jovic, M., Naslavsky, N., Caplan, S., Sorgen, P.L.
J. Biomol. NMR (2007), 39, 323-329, PubMed 17899392 , DOI 10.1007/s10858-007-9196-0 , Abstract

Related entities 1. EH domain of EHD1, : 1 : 2 : 35 entities Detail

Interaction partners 1. EH domain of EHD1, : 27 interactors Detail

More details for 27 interactors identified by 16 citations

Experiments performed 11 experiments Detail

1 3D HNCACB