BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.3 % (1322 of 1549)	89.9 % (727 of 809)	76.1 % (455 of 598)	98.6 % (140 of 142)
Backbone	84.4 % (655 of 776)	97.4 % (260 of 267)	70.5 % (270 of 383)	99.2 % (125 of 126)
Sidechain	88.1 % (788 of 894)	86.3 % (468 of 542)	90.8 % (305 of 336)	93.8 % (15 of 16)
Aromatic	73.1 % (76 of 104)	88.5 % (46 of 52)	56.0 % (28 of 50)	100.0 % (2 of 2)
Methyl	95.5 % (147 of 154)	94.8 % (73 of 77)	96.1 % (74 of 77)

1. C2 domain of Smurf2

GPVKLRLTVL CAKNLVKKDF FRLPDPFAKV VVDGSGQCHS TDTVKNTLDP KWNQHYDLYI GKSDSVTISV WNHKKIHKKQ GAGFLGCVRL LSNAINRLKD TGYQRLDLCK LGPNDNDTVR GQIVVSLQSR D

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details C2 domain 13C, 15N

#	Name	Isotope labeling	Concentration
5	hepes	[U-13C; U-15N]	20 mM
6	sodium chloride	natural abundance	200 mM
7	DTT	natural abundance	3 mM
8	sodium azide	natural abundance	0.03 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772925 ppm	internal	direct	0.2514495
¹H	water	protons	4.772925 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772925 ppm	internal	direct	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772925 ppm	internal	direct	0.2514495
¹H	water	protons	4.772925 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772925 ppm	internal	direct	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772925 ppm	internal	direct	0.2514495
¹H	water	protons	4.772925 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772925 ppm	internal	direct	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JQZ, Strand ID: A Detail

Release date

2008-02-27

Citation

Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain
Wiesner, S., Ogunjimi, A.A., Wang, H., Rotin, D., Sicheri, F., Wrana, J.L., Forman-Kay, J.D.
Cell (2007), 130, 651-662, PubMed 17719543 , DOI 10.1016/j.cell.2007.06.050 , Abstract

Related entities 1. C2 domain of Smurf2, : 1 : 4 : 77 entities Detail

Interaction partners 1. C2 domain of Smurf2, : 76 interactors Detail

More details for 76 interactors identified by 12 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details C2 domain 13C, 15N

#	Name	Isotope labeling	Concentration
5	hepes	[U-13C; U-15N]	20 mM
6	sodium chloride	natural abundance	200 mM
7	DTT	natural abundance	3 mM
8	sodium azide	natural abundance	0.03 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

4 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

5 3D HNCA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

6 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

7 3D HN(CO)CA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

8 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.8 mM C2 domain

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	3 mM
4	sodium azide	natural abundance	0.03 %

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details C2 domain 13C, 15N

#	Name	Isotope labeling	Concentration
5	hepes	[U-13C; U-15N]	20 mM
6	sodium chloride	natural abundance	200 mM
7	DTT	natural abundance	3 mM
8	sodium azide	natural abundance	0.03 %

nullKeywords C2 domain, Phospholipid binding, Smurf2, Ubiquitin protein ligase

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BMRB: 15306

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. C2 domain of Smurf2, : 1 : 4 : 77 entities Detail

Interaction partners 1. C2 domain of Smurf2, : 76 interactors Detail

Experiments performed 10 experiments Detail

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