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BMRB: 15313

2KN6

1H, 15N and 13C backbone and side chain chemical shifts of human ASC (apoptosis-associated speck-like protein containing a CARD domain)

Authors

de Alba, E.

Assembly

single polypeptide chain

Entity

1. single polypeptide chain (polymer, Thiol state: all free), 215 monomers, 23789.85 Da Detail

MGSSHHHHHH SSGLVPRGSH MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYGKV LTDEQYQAVR AEPTNPSKMR KLFSFTPAWN WTCKDLLLQA LRESQSYLVE DLERS

Formula weight

23789.85 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 85.7 %, Completeness (bb): 89.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (2115 of 2469)	85.0 % (1088 of 1280)	85.3 % (823 of 965)	91.1 % (204 of 224)
Backbone	89.8 % (1141 of 1270)	89.4 % (389 of 435)	89.5 % (564 of 630)	91.7 % (188 of 205)
Sidechain	82.6 % (1156 of 1399)	82.7 % (699 of 845)	82.4 % (441 of 535)	84.2 % (16 of 19)
Aromatic	49.4 % (86 of 174)	50.6 % (44 of 87)	46.4 % (39 of 84)	100.0 % (3 of 3)
Methyl	96.5 % (245 of 254)	97.6 % (124 of 127)	95.3 % (121 of 127)

1. ASC

Show sample condition

Sample #1

Solvent system 5% D2O, Pressure 1 atm, Temperature 298 K, pH 3.8

#	Name	Isotope labeling	Type	Concentration
1	ASC	[U-99% 13C; U-99% 15N]		0.2 mM
2	TCEP	[U-99% 2H]		5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 3.8

#	Name	Isotope labeling	Type	Concentration
3	ASC	[U-99% 13C; U-99% 15N]		0.2 mM
4	TCEP	[U-99% 2H]		5 mM

Sample #3

Solvent system 5% D2O, Pressure 1 atm, Temperature 298 K, pH 3.8

#	Name	Isotope labeling	Type	Concentration
5	ASC	[U-99% 15N]		0.2 mM
6	TCEP	[U-99% 2H]		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	external	indirect	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	urea	nitrogen	external	indirect	1.0

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	external	indirect	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	urea	nitrogen	external	indirect	1.0

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	external	indirect	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	urea	nitrogen	external	indirect	1.0

Referencing offset: 0.0 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	external	indirect	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	urea	nitrogen	external	indirect	1.0

Referencing offset: -0.31 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KN6, Strand ID: A Detail

Release date

2007-08-21

Citation

1H, 15N and 13C backbone and side chain chemical shifts of human ASC (apoptosis-associated speck-like protein containing a CARD domain)
de Alba, E.
Biomol. NMR Assign. (2007), 1, 135-137, PubMed 19636848 , DOI 10.1007/s12104-007-9036-x , Abstract

Related entities 1. single polypeptide chain, : 1 : 2 : 16 entities Detail

Interaction partners 1. single polypeptide chain, : 29 interactors Detail

More details for 29 interactors identified by 15 citations