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BMRB: 15324

2JRA

A novel domain-swapped solution NMR structure of protein RPA2121 from Rhodopseudomonas palustris

Authors

Wu, B., Yee, A., Lemak, A., Cort, J., Bansal, S., Semest, A., Guido, V., Kennedy, M.A., Prestegard, J.H., Arrowsmith, C.

Assembly

RPA2121 doamin-swapped dimer

Entity

1. RPA2121 doamin-swapped dimer (polymer, Thiol state: not present), 67 monomers, 7285.157 × 2 Da Detail

MMTASDRLGA DPTQAASSPG GARAVSIVGN QIDSRELFTV DREIVIAHGD DRYRLRLTSQ NKLILTK

Total weight

14570.314 Da

Max. entity weight

7285.157 Da

Source organism

Rhodopseudomonas palustris

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 93.9 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (694 of 739)	94.3 % (361 of 383)	93.7 % (268 of 286)	92.9 % (65 of 70)
Backbone	95.2 % (379 of 398)	97.1 % (133 of 137)	93.9 % (184 of 196)	95.4 % (62 of 65)
Sidechain	93.1 % (375 of 403)	92.7 % (228 of 246)	94.7 % (144 of 152)	60.0 % (3 of 5)
Aromatic	81.8 % (18 of 22)	100.0 % (11 of 11)	63.6 % (7 of 11)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. unknown function protein RPA2121

MMTASDRLGA DPTQAASSPG GARAVSIVGN QIDSRELFTV DREIVIAHGD DRYRLRLTSQ NKLILTK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	unknown function protein RPA2121	[U-99% 13C; U-99% 15N]	0.5 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	sodium azide	natural abundance	0.01 %
5	benzamidine	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	unknown function protein RPA2121	[U-7% 13C; U-99% 15N]	0.5 mM
7	TRIS	[U-100% 2H]	10 mM
8	sodium chloride	natural abundance	300 mM
9	sodium azide	natural abundance	0.01 %
10	benzamidine	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JRA, Strand ID: A, B Detail

Release date

2009-10-08

Citation

A novel domain-swapped solution NMR structure of protein RPA2121 from Rhodopseudomonas palustris
Wu, B., Yee, A., Lemak, A., Cort, J., Bansal, S., Semest, A., Guido, V., Kennedy, M.A., Prestegard, J.H., Arrowsmith, C.
Not known

Related entities 1. RPA2121 doamin-swapped dimer, : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCO