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BMRB: 15329

2JRF

Solution NMR structure of Tubulin polymerization-promoting protein family member 3 from Homo sapiens. Northeast Structural Genomics target HR387.

Authors

Aramini, J.M., Rossi, P., Shastry, R., Nwosu, C., Cunningham, K., Xiao, R., Liu, J., Baran, M.C., Rajan, P.K., Acton, T.B., Rost, B., Montelione, G.T.

Assembly

hr387

Entity

1. hr387 (polymer, Thiol state: all free), 184 monomers, 20032.28 Da Detail

MAASTDIAGL EESFRKFAIH GDPKASGQEM NGKNWAKLCK DCKVADGKSV TGTDVDIVFS KVKGKSARVI NYEEFKKALE ELATKRFKGK SKEEAFDAIC QLVAGKEPAN VGVTKAKTGG AVDRLTDTSR YTGSHKERFD ESGKGKGIAG RQDILDDSGY VSAYKNAGTY DAKVKKLEHH HHHH

Formula weight

20032.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 89.7 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.7 % (1864 of 2077)	86.7 % (938 of 1082)	93.0 % (748 of 804)	93.2 % (178 of 191)
Backbone	94.1 % (1035 of 1100)	93.0 % (358 of 385)	95.1 % (507 of 533)	93.4 % (170 of 182)
Sidechain	86.3 % (986 of 1142)	83.2 % (580 of 697)	91.3 % (398 of 436)	88.9 % (8 of 9)
Aromatic	74.0 % (114 of 154)	74.0 % (57 of 77)	73.7 % (56 of 76)	100.0 % (1 of 1)
Methyl	98.8 % (166 of 168)	97.6 % (82 of 84)	100.0 % (84 of 84)

1. hr387

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	hr387	[U-100% 13C; U-100% 15N]	1.04 mM
2	ammonium acetate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	95 %
8	D2O		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	hr387	[U-5% 13C; U-100% 15N]	0.7 mM
10	ammonium acetate	natural abundance	20 mM
11	calcium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	calcium chloride	natural abundance	5 mM
14	sodium azide	natural abundance	0.02 %
15	H2O	natural abundance	95 %
16	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JRF, Strand ID: A Detail

Release date

2007-07-18

Citation

Solution NMR structure of Tubulin polymerization-promoting protein family member 3 from Homo sapiens. Northeast Structural Genomics target HR387
Aramini, J.M., Rossi, P., Shastry, R., Nwosu, C., Cunningham, K., Xiao, R., Liu, J., Baran, M.C., Rajan, P.K., Acton, T.B., Rost, B., Montelione, G.T.

Related entities 1. hr387, : 1 : 29 entities Detail

Interaction partners 1. hr387, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC