NMR SOLUTION STRUCTURE OF THE ANTICODON OF E.coli TRNA-VAL3 WITH 2 MODIFICATIONS (cmo5U34 M6A37)
Polymer type: polyribonucleotide
Total | 1H | |
---|---|---|
All | 72.4 % (97 of 134) | 72.4 % (97 of 134) |
Suger, PO4 | 66.7 % (60 of 90) | 66.7 % (60 of 90) |
Nucleobase | 84.1 % (37 of 44) | 84.1 % (37 of 44) |
Aromatic | 96.9 % (31 of 32) | 96.9 % (31 of 32) |
1. RNA ASLval
CCUCCCUXAC XAGGAGGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
2 | H2O | 90 % | ||
3 | D2O | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 275 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
2 | H2O | 90 % | ||
3 | D2O | 10 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA 17mer | natural abundance | 1.5 ~ 2.0 mM | |
5 | D2O | 100 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_15331_2jrg.nef |
Input source #2: Coordindates | 2jrg.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | True (see coodinates for details) |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
Ptnr_site_1 | Ptnr_site_2 | Redox_state_prediction_1 | Redox_state_prediction_2 | Distance (Å) |
---|---|---|---|---|
1:7:U:O3' | 1:8:CM0:P | unknown | unknown | n/a |
1:8:CM0:O3' | 1:9:A:P | unknown | unknown | n/a |
1:10:C:O3' | 1:11:6MZ:P | unknown | unknown | n/a |
1:11:6MZ:O3' | 1:12:A:P | unknown | unknown | n/a |
Non-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
A | 34 | CM0 | 5-(CARBOXYMETHOXY) URIDINE-5'-MONOPHOSPHATE | Assigned chemical shifts, Distance restraints, Torsion angle restraints, Coordinates |
A | 37 | 6MZ | N6-METHYLADENOSINE-5'-MONOPHOSPHATE | Assigned chemical shifts, Distance restraints, Torsion angle restraints, Coordinates |
Sequence alignments
--30--------40--- CCUCCCUXACXAGGAGG ||||||||||||||||| CCUCCCUXACXAGGAGG --------10-------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 17 | 0 | 0 | 100.0 |
Content subtype: combined_15331_2jrg.nef
Assigned chemical shifts
Comp_index_ID | Comp_ID |
---|---|
34 | CM0 |
37 | 6MZ |
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
39 | G | H21 | 6.222 |
39 | G | H22 | 8.282 |
40 | G | H21 | 5.946 |
40 | G | H22 | 8.049 |
41 | A | H61 | 7.868 |
41 | A | H62 | 6.652 |
42 | G | H21 | 6.127 |
42 | G | H22 | 8.531 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 134 | 97 | 72.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 90 | 60 | 66.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 44 | 37 | 84.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 10 | 10 | 100.0 |
Covalent bonds
Distance restraints
--30--------40--- CCUCCCUXACXAGGAGG ||||| ||||| CCUCC.......GGAGG
Dihedral angle restraints
--30--------40--- CCUCCCUXACXAGGAGG ||||||||||||||||| CCUCCCUXACXAGGAGG