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BMRB: 15344

2JRT

The solution structure of the protein coded by gene RHOS4_12090 of R. sphaeroides. Northeast structural genomics target RhR5

Authors

Wang, L., Chen, C., Nwosu, C., Cunningham, K., Owens, L., Ma, L., Xiao, R., Liu, J., Baran, M.C., Swapna, G., Acton, T.B., Rost, B., Montelione, G.

Assembly

RhR5

Entity

1. RhR5 (polymer, Thiol state: not present), 95 monomers, 10856.42 Da Detail

MYLKRVDGPR QVTLPDGTVL SRADLPPLDT RRWVASRKAA VVKAVIHGLI TEREALDRYS LSEEEFALWR SAVAAHGEKA LKVTMIQKYR QLHHH

Formula weight

10856.42 Da

Source organism

Cereibacter sphaeroides

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 93.2 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1045 of 1121)	92.1 % (537 of 583)	94.1 % (416 of 442)	95.8 % (92 of 96)
Backbone	95.6 % (537 of 562)	93.2 % (177 of 190)	97.2 % (273 of 281)	95.6 % (87 of 91)
Sidechain	91.8 % (597 of 650)	91.6 % (360 of 393)	92.1 % (232 of 252)	100.0 % (5 of 5)
Aromatic	82.1 % (64 of 78)	84.6 % (33 of 39)	78.4 % (29 of 37)	100.0 % (2 of 2)
Methyl	98.4 % (122 of 124)	98.4 % (61 of 62)	98.4 % (61 of 62)

1. RhR5

MYLKRVDGPR QVTLPDGTVL SRADLPPLDT RRWVASRKAA VVKAVIHGLI TEREALDRYS LSEEEFALWR SAVAAHGEKA LKVTMIQKYR QLHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.0 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RhR5	[U-5% 13C; U-100% 15N]	1.0 mM
2	DTT	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.0 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	RhR5	[U-100% 13C; U-100% 15N]	1.0 mM
5	DTT	natural abundance	10 mM
6	sodium chloride	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JRT, Strand ID: A Detail

Release date

2008-02-20

Citation

The solution structure of the protein coded by gene RHOS4_12090 of R. sphaeroides. Northeast structural genomics target RhR5
Wang, L., Montelione, G.
Proteins

Related entities 1. RhR5, : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC