BMRB: 15345

Solution structure of Blo t 5

Authors

Chan, S., Mok, Y.

Assembly

Blo t 5

Entity

1. Blo t 5 (polymer, Thiol state: all free), 112 monomers, 13271.83 Da Detail

KKDDFRNEFD HLLIEQANHA IEKGEHQLLY LQHQLDELNE NKSKELQEKI IRELDVVCAM IEGAQGALER ELKRTDLNIL ERFNYEEAQT LSKILLKDLK ETEQKVKDIQ TQ

Formula weight

13271.83 Da

Source organism

Blomia tropicalis

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 66.4 %, Completeness (bb): 77.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	66.4 % (920 of 1386)	61.9 % (454 of 734)	68.5 % (359 of 524)	83.6 % (107 of 128)
Backbone	77.8 % (523 of 672)	87.7 % (199 of 227)	65.5 % (218 of 333)	94.6 % (106 of 112)
Sidechain	60.6 % (499 of 823)	50.3 % (255 of 507)	81.0 % (243 of 300)	6.3 % (1 of 16)
Aromatic	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)
Methyl	73.5 % (97 of 132)	63.6 % (42 of 66)	83.3 % (55 of 66)

1. Blo t 5

KKDDFRNEFD HLLIEQANHA IEKGEHQLLY LQHQLDELNE NKSKELQEKI IRELDVVCAM IEGAQGALER ELKRTDLNIL ERFNYEEAQT LSKILLKDLK ETEQKVKDIQ TQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Blo t 5	[U-99% 13C; U-99% 15N]		2 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
2	Blo t 5	[U-100% 13C]		2 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	Blo t 5	[U-99% 15N]		2 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2JRK, Strand ID: A Detail

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Release date

2008-08-05

Citation

Nuclear magnetic resonance structure and IgE epitopes of Blo t 5, a major dust mite allergen
Chan, S., Ong, T., Gao, Y., Tiong, Y., Wang, D., Chew, F., Mok, Y.
J. Immunol. (2008), 181, 2586-2596, PubMed 18684949 , DOI: , Abstract

Related entities 1. Blo t 5, : 1 : 3 : 7 entities Detail

Experiments performed 7 experiments Detail