BMRBj - BMREntryMaster

Found 1 Document (0.645 seconds)

BMRB: 15386

2JT1

Solution NMR structure of PefI protein from Salmonella typhimurium. Northeast Structural Genomics target StR82.

Authors

Aramini, J.M., Rossi, P., Wang, H., Nwosu, C., Cunningham, K., Ma, L., Xiao, R., Liu, J., Baran, M.C., Swapna, G.VT., Acton, T.B., Rost, B., Montelione, G.T.

Assembly

str82

Entity

1. str82 (polymer, Thiol state: all free), 77 monomers, 8671.794 Da Detail

MSESIVTKII SIVQERQNMD DGAPVKTRDI ADAAGLSIYQ VRLYLEQLHD VGVLEKVNAG KGVPGLWRLL EHHHHHH

Formula weight

8671.794 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 88.0 %, Completeness (bb): 90.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.0 % (785 of 892)	86.1 % (397 of 461)	89.7 % (313 of 349)	91.5 % (75 of 82)
Backbone	90.2 % (413 of 458)	88.6 % (140 of 158)	91.1 % (205 of 225)	90.7 % (68 of 75)
Sidechain	86.3 % (436 of 505)	84.8 % (257 of 303)	88.2 % (172 of 195)	100.0 % (7 of 7)
Aromatic	53.6 % (30 of 56)	53.6 % (15 of 28)	51.9 % (14 of 27)	100.0 % (1 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. Str82

MSESIVTKII SIVQERQNMD DGAPVKTRDI ADAAGLSIYQ VRLYLEQLHD VGVLEKVNAG KGVPGLWRLL EHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Str82	[U-100% 13C; U-100% 15N]	0.72 mM
2	ammonium acetate	natural abundance	20 mM
3	sodium chloride	natural abundance	450 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %
7	H2O		95 %
8	D2O		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Str82	[U-5% 13C; U-100% 15N]	0.46 mM
10	ammonium acetate	natural abundance	20 mM
11	sodium chloride	natural abundance	450 mM
12	DTT	natural abundance	10 mM
13	calcium chloride	natural abundance	5 mM
14	sodium azide	natural abundance	0.02 %
15	H2O		95 %
16	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.4 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JT1, Strand ID: A Detail

Release date

2007-08-12

Citation

Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar Typhimurium
Aramini, J.M., Rossi, P., Cort, J.R., Ma, L., Xiao, R., Acton, T.B., Montelione, G.T.
Proteins (2011), 79, 335-339, PubMed 20979070 , DOI 10.1002/prot.22869 , Abstract

Related entities 1. str82, : 1 : 5 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC