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BMRB: 15393

2JSS

Structure of Chz1 Complexed with H2A.Z-H2B and Eviction of Nucleosomal H2A-H2B

Authors

Zhou, Z., Feng, H., Hansen, F.D., Luk, E., Kato, H., Freedberg, D., Key, L.E., Wu, C., Bai, Y.

Assembly

H2A.Z-H2B-Chz1 complex

Entity

1. H2A.Z-H2B (polymer, Thiol state: all free), 200 monomers, 21981.93 Da Detail

RKETYSSYIY KVLKQTHPDT GISQKSMSIL NSFVNDIFER IATEASKLAA YNKKSTISAR EIQTAVRLIL PGELAKHAVS EGTRAVTKYS SSTQAQSSSA RAGLQFPVGR IKRYLKRHAT GRTRVGSKAA IYLTAVLEYL TAEVLELAGN AAKDLKVKRI TPRHLQLAIR GDDELDSLIR ATIASGGVLP HIAEELDKKE

2. Chz1 (polymer, Thiol state: all free), 62 monomers, 6950.372 Da Detail

TVEDSESDMD DAKLDALMGN EGEEEEDDLA EIDTSNIITS GRRTRGKVID YKKTAEELDK KE

Total weight

28932.3 Da

Max. entity weight

21981.93 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 81.8 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (2435 of 2977)	76.5 % (1184 of 1548)	86.1 % (998 of 1159)	93.7 % (253 of 270)
Backbone	95.8 % (1497 of 1562)	94.6 % (505 of 534)	96.4 % (743 of 771)	96.9 % (249 of 257)
Sidechain	70.5 % (1171 of 1662)	66.6 % (675 of 1014)	77.3 % (491 of 635)	38.5 % (5 of 13)
Aromatic	21.3 % (26 of 122)	42.6 % (26 of 61)	0.0 % (0 of 61)
Methyl	88.4 % (283 of 320)	93.1 % (149 of 160)	83.7 % (134 of 160)

1. H2A.Z-H2B

2. Chz1

TVEDSESDMD DAKLDALMGN EGEEEEDDLA EIDTSNIITS GRRTRGKVID YKKTAEELDK KE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013292

Referencing offset: 0.8 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013292

Referencing offset: 0.8 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013292

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013292

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JSS, Strand ID: A, B Detail

Release date

2008-07-28

Citation

NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B
Zhou, Z., Feng, H., Hansen, F., Kato, H., Luk, E., Freedberg, D.I., Key, L.E., Wu, C., Bai, Y.
Nat. Struct. Mol. Biol. (2008), 15, 868-869, PubMed 18641662 , DOI 10.1038/nsmb.1465 , Abstract

Related entities 1. H2A.Z-H2B, : 8 entities Detail

Related entities 2. Chz1, : 1 : 3 : 2 entities Detail

Interaction partners 2. Chz1, : 20 interactors Detail

More details for 20 interactors identified by 4 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

2 2D 1H-1H NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

3 3D CBCA(CO)NH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

4 3D CBCA(CO)NH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

5 3D HNCACB

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

6 3D HNCACB

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

7 3D HNCO

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

8 3D HNCO

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

9 3D HNCA

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

10 3D HN(CO)CA

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

11 3D H(CCO)NH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

12 3D HCCH-TOCSY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

13 3D HBHA(CO)NH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

14 3D HNHA

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

15 3D HNCANNH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

16 3D TOCSY-HSQC

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

17 3D 1H-15N NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

18 3D 1H-15N NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

19 3D 1H-13C NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

20 3D 1H-13C NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker DRX (700 MHz)

Sample #1

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2A.Z-H2B	[U-35% 2H]	1.0 ~ 1.2 mM
2	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
3	MES		25 mM
4	KCl		200 mM
5	EDTA		1 mM

Sample #2

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	H2A.Z-H2B	[U-100% 15N; U-100% 2H]	1.0 ~ 1.2 mM
7	Chz1	[U-30%2H]	1.0 ~ 1.2 mM
8	MES		25 mM
9	KCl		200 mM
10	EDTA		1 mM

Sample #3

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	H2A.Z-H2B	[U-100% 13C; U-30% 2H]	1.0 ~ 1.2 mM
12	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
13	MES		25 mM
14	KCl		200 mM
15	EDTA		1 mM

Sample #4

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	H2A.Z-H2B	[U-13C; U-15N; U-2H]	1.0 ~ 1.2 mM
17	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
18	MES		25 mM
19	KCl		200 mM
20	EDTA		1 mM

Sample #5

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	H2A.Z-H2B	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
22	Chz1	[U-100% 2H]	1.0 ~ 1.2 mM
23	MES		25 mM
24	KCl		200 mM
25	EDTA		1 mM

Sample #6

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
26	H2A.Z-H2B	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
27	Chz1	[U-100% 13C;U-35%2H]/[U-100% 2H]	1.0 ~ 1.2 mM
28	MES		25 mM
29	KCl		200 mM
30	EDTA		1 mM

Sample #7

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
31	H2A.Z-H2B	[U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
32	Chz1	[U-30% 2H]	1.0 ~ 1.2 mM
33	MES		25 mM
34	KCl		200 mM
35	EDTA		1 mM

Sample #8

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
36	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
37	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
38	MES		25 mM
39	KCl		200 mM
40	EDTA		1 mM

Sample #9

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
41	Chz1	[U-100% 15N; U-30%2H]	1.0 ~ 1.2 mM
42	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
43	MES		25 mM
44	KCl		200 mM
45	EDTA		1 mM

Sample #10

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
46	Chz1	[U-100% 13C; U-35%2H]	1.0 ~ 1.2 mM
47	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
48	MES		25 mM
49	KCl		200 mM
50	EDTA		1 mM

Sample #11

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
51	Chz1	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
52	H2A.Z-H2B	[U-100%15N; U-100% 2H]	1.0 ~ 1.2 mM
53	MES		25 mM
54	KCl		200 mM
55	EDTA		1 mM

Sample #12

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
56	H2A.Z-H2B	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
57	Chz1	[U-100% 13C; U-100% 15N; U-100%2H]	1.0 ~ 1.2 mM
58	MES		25 mM
59	KCl		200 mM
60	EDTA		1 mM

Sample #13

Solvent system 90% H2O/10% D2O or 100%D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
61	Chz1	[U-10% 13C; U-100% 15N]	1.0 ~ 1.2 mM
62	H2A.Z-H2B	[U-100% 2H]	1.0 ~ 1.2 mM
63	MES		25 mM
64	KCl		200 mM
65	EDTA		1 mM

NMR combined restraints 11 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_15393_2jss.nef
Input source #2: Coordindates	2jss.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSA
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSA

-------110-------120-------130-------140-------150-------160-------170-------180-------190--
RAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30--------40--------50--------60--
TVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
TVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	192	0	0	100.0
B	B	62	0	0	100.0

Content subtype: combined_15393_2jss.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	2369		100.0 (chain: A, length: 192), 100.0 (chain: B, length: 62)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1844 (1)	noe	98.4 (chain: A, length: 192)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	597 (1)	noe	4.2 (chain: A, length: 192), 98.4 (chain: B, length: 62)
3	Distance restraints	CNS/XPLOR_distance_constraints_10	Warning	2486 (1)	noe	99.5 (chain: A, length: 192), 19.4 (chain: B, length: 62)
4	Distance restraints	CNS/XPLOR_distance_constraints_11	Warning	152 (1)	noe	31.8 (chain: A, length: 192)
5	Distance restraints	CNS/XPLOR_distance_constraints_7	Warning	542 (1)	noe	9.4 (chain: A, length: 192), 98.4 (chain: B, length: 62)
6	Distance restraints	CNS/XPLOR_distance_constraints_6	Warning	20 (1)	hbond	29.0 (chain: B, length: 62)
7	Distance restraints	CNS/XPLOR_distance_constraints_13	OK	180 (1)	hbond	63.0 (chain: A, length: 192)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	Warning	22 (22)	.	25.8 (chain: B, length: 62)
2	Dihedral angle restraints	CNS/XPLOR_dihedral_8	OK	218 (218)	.	74.0 (chain: A, length: 192)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSA
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSA

-------110-------120-------130-------140-------150-------160-------170-------180-------190--
RAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI

Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--
TVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
TVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE

Total number of assignments
- ¹³C chemical shifts: 979
- ¹H chemical shifts: 1139
- ¹⁵N chemical shifts: 251

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
6	SER	HG	5.685
23	SER	HG	5.703
26	SER	HG	5.708
28	SER	HG	5.693
46	SER	HG	5.455
55	SER	HG	5.664
58	SER	HG	5.693
90	SER	HG	5.54
91	SER	HG	5.67
92	SER	HG	5.71
97	SER	HG	5.69
98	SER	HG	5.7
127	SER	HG	5.69
177	SER	HG	5.879

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	865	733	84.7
¹H chemical shifts	1143	863	75.5
¹⁵N chemical shifts	213	186	87.3

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	384	374	97.4
¹H chemical shifts	390	377	96.7
¹⁵N chemical shifts	187	184	98.4

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	481	359	74.6
¹H chemical shifts	753	486	64.5
¹⁵N chemical shifts	26	2	7.7

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	129	102	79.1
¹H chemical shifts	129	123	95.3

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	57	0	0.0
¹H chemical shifts	57	24	42.1

Entity_assemble_ID: B

Peptide bond of PRO
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A
- Chain_ID: B

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 98.4%
- Total number of restraints: 1841
- Weight of restraints: 1.0 (1841)
- Potential type of restraints: square-well-parabolic (1841)
- Range of distance target values: 1.4, 4.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
    None
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 4.2%
    - Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 98.4%
  - Total number of restraints: 596
  - Weight of restraints: 1.0 (596)
  - Potential type of restraints: square-well-parabolic (596)
  - Range of distance target values: 2.25, 5.15 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_10
    - Status: Warning
    - Experiment type: noe
    - Sequence coverage of experimental data
      - Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 99.5%
      - Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 19.4%
    - Total number of restraints: 2486
    - Weight of restraints: 1.0 (2486)
    - Potential type of restraints: square-well-parabolic (2486)
    - Range of distance target values: 2.4, 4.9 (Å)
    - Histogram of distance target values
    - Histogram of discrepancy in distance target values
    - Distance restraints per residue
      - Chain_ID: A
      - Chain_ID: B
    - Distance restraints on contact map
    - Saveframe: CNS/XPLOR_distance_constraints_11
      - Status: Warning
      - Experiment type: noe
      - Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 31.8%
      - Total number of restraints: 152
        Intra-residue restraints, i = j : 16
        Sequential restraints, | i - j | = 1 : 17
        Backbone-side chain: 2
        Side chain-side chain: 15
        Medium range restraints, 1 < | i - j | < 5 : 32
        Backbone-side chain: 7
        Side chain-side chain: 25
        Long range restraints, | i - j | >= 5 : 87
      - Weight of restraints: 1.0 (152)
        Intra-residue restraints, i = j : 1.0 (16)
        Sequential restraints, | i - j | = 1 : 1.0 (17)
        Backbone-side chain: 1.0 (2)
        Side chain-side chain: 1.0 (15)
        Medium range restraints, 1 < | i - j | < 5 : 1.0 (32)
        Backbone-side chain: 1.0 (7)
        Side chain-side chain: 1.0 (25)
        Long range restraints, | i - j | >= 5 : 1.0 (87)
      - Potential type of restraints: square-well-parabolic (152)
        Intra-residue restraints, i = j : square-well-parabolic (16)
        Sequential restraints, | i - j | = 1 : square-well-parabolic (17)
        Backbone-side chain: square-well-parabolic (2)
        Side chain-side chain: square-well-parabolic (15)
        Medium range restraints, 1 < | i - j | < 5 : square-well-parabolic (32)
        Backbone-side chain: square-well-parabolic (7)
        Side chain-side chain: square-well-parabolic (25)
        Long range restraints, | i - j | >= 5 : square-well-parabolic (87)
      - Range of distance target values: 2.4, 4.9 (Å)
      - Histogram of distance target values
      - Histogram of discrepancy in distance target values
      - Distance restraints per residue
        Chain_ID: A
        Chain_ID: B
        None
      - Distance restraints on contact map
      - Saveframe: CNS/XPLOR_distance_constraints_7
        Status: Warning
        Experiment type: noe
        Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 9.4%
        Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 98.4%
        --------10--------20--------30--------40--------50--------60-- TVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .VEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKE
        Total number of restraints: 541
        Intra-residue restraints, i = j : 230
        Sequential restraints, | i - j | = 1 : 200
        Backbone-backbone: 85
        Backbone-side chain: 115
        Medium range restraints, 1 < | i - j | < 5 : 69
        Backbone-backbone: 34
        Backbone-side chain: 35
        Long range restraints, | i - j | >= 5 : 2
        Inter-chain restraints: 19
        Symmetric restraints: 21
        Weight of restraints: 1.0 (541)
        Intra-residue restraints, i = j : 1.0 (230)
        Sequential restraints, | i - j | = 1 : 1.0 (200)
        Backbone-backbone: 1.0 (85)
        Backbone-side chain: 1.0 (115)
        Medium range restraints, 1 < | i - j | < 5 : 1.0 (69)
        Backbone-backbone: 1.0 (34)
        Backbone-side chain: 1.0 (35)
        Long range restraints, | i - j | >= 5 : 1.0 (2)
        Inter-chain restraints: 1.0 (19)
        Symmetric restraints: 1.0 (21)
        Potential type of restraints: square-well-parabolic (541)
        Intra-residue restraints, i = j : square-well-parabolic (230)
        Sequential restraints, | i - j | = 1 : square-well-parabolic (200)
        Backbone-backbone: square-well-parabolic (85)
        Backbone-side chain: square-well-parabolic (115)
        Medium range restraints, 1 < | i - j | < 5 : square-well-parabolic (69)
        Backbone-backbone: square-well-parabolic (34)
        Backbone-side chain: square-well-parabolic (35)
        Long range restraints, | i - j | >= 5 : square-well-parabolic (2)
        Inter-chain restraints: square-well-parabolic (19)
        Symmetric restraints: square-well-parabolic (21)
        Range of distance target values: 2.4, 4.9 (Å)
        Histogram of distance target values
        Histogram of discrepancy in distance target values
        Distance restraints per residue
        Chain_ID: A
        Chain_ID: B
        Distance restraints on contact map
        Chain_ID_1: B - Chain_ID_2: B
        Saveframe: CNS/XPLOR_distance_constraints_6
        Status: Warning
        Experiment type: hbond
        Sequence coverage of experimental data
        Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 29.0%
        Total number of restraints: 20
        Total hydrogen bond restraints: 20
        O...H-x: 10
        O...h-N: 10
        Weight of restraints: 1.0 (20)
        Total hydrogen bond restraints: 1.0 (20)
        O...H-x: 1.0 (10)
        O...h-N: 1.0 (10)
        Potential type of restraints: square-well-parabolic (20)
        Total hydrogen bond restraints: square-well-parabolic (20)
        O...H-x: square-well-parabolic (10)
        O...h-N: square-well-parabolic (10)
        Range of distance target values: 1.9, 2.75 (Å)
        Histogram of distance target values
        Distance restraints per residue
        Chain_ID: A
        None
        Chain_ID: B
        Distance restraints on contact map
        Saveframe: CNS/XPLOR_distance_constraints_13
        Status: OK
        Experiment type: hbond
        Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 63.0%
        --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSA ||||||||||| |||||||||||||||||||||||||||| ||||||||||| |||||||||||||||||||| | .....SSYIYKVLKQT.......QKSMSILNSFVNDIFERIATEASKLAAY.......AREIQTAVRLI..GELAKHAVSEGTRAVTKYSS......S.. --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180-------190-- RAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI | ||||||||||| ||||||||||||||||||||||||| ||||||||| || || .A....PVGRIKRYLKR...........AAIYLTAVLEYLTAEVLELAGNAAK.........RHLQLAIRG.DE..SL -------110-------120-------130-------140-------150-------160-------170--------
        Total number of restraints: 180
        Total hydrogen bond restraints: 180
        O...H-x: 90
        O...h-N: 90
        Weight of restraints: 1.0 (180)
        Total hydrogen bond restraints: 1.0 (180)
        O...H-x: 1.0 (90)
        O...h-N: 1.0 (90)
        Potential type of restraints: square-well-parabolic (180)
        Total hydrogen bond restraints: square-well-parabolic (180)
        O...H-x: square-well-parabolic (90)
        O...h-N: square-well-parabolic (90)
        Range of distance target values: 1.9, 2.7 (Å)
        Histogram of distance target values
        Distance restraints per residue
        Chain_ID: A
        Chain_ID: B
        None
        Distance restraints on contact map
        Chain_ID_1: A - Chain_ID_2: A

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 62, Sequence coverage: 25.8%
- Total number of restraints: 22
- Total number of restraints per polymer type: 22
- Weight of restraints: 1.0 (22)
- Potential type of restraints: square-well-parabolic (22)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
- Saveframe: CNS/XPLOR_dihedral_8
  - Status: OK
  - Experiment type: .
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 192, Sequence coverage: 74.0%
  - Total number of restraints: 218
  - Total number of restraints per polymer type: 218
  - Weight of restraints: 1.0 (218)
  - Potential type of restraints: square-well-parabolic (218)
  - Plot of Phi-Psi angle restraints
  - Dihedral angle restraints per residue
    - Chain_ID: A

Keywords Histone/chaperone complex, intrinsically unfolded protein

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	124	123	99.2
¹H chemical shifts	128	122	95.3
¹⁵N chemical shifts	62	61	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	134	123	91.8
¹H chemical shifts	225	136	60.4
¹⁵N chemical shifts	5	4	80.0

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Found 1 Document (1.284 seconds)

BMRB: 15393

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. H2A.Z-H2B, : 8 entities Detail

Related entities 2. Chz1, : 1 : 3 : 2 entities Detail

Interaction partners 2. Chz1, : 20 interactors Detail

Experiments performed 20 experiments Detail

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