BMRBj - BMREntryMaster

Found 1 Document (0.49 seconds)

BMRB: 15430

2JU4

The chemical shift assignment of gamma subunit of phosphodiesterase

Authors

Song, J., Guo, L., Ruoho, A.E., Markley, J.L.

Assembly

PDEgamma

Entity

1. PDEgamma (polymer, Thiol state: not present), 87 monomers, 9637.041 Da Detail

MNLEPPKAEI RSATRVMGGP VTPRKGPPKF KQRQTRQFKS KPPKKGVQGF GDDIPGMEGL GTDITVIAPW EAFNHLELHE LAQYGII

Formula weight

9637.041 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 75.8 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.8 % (787 of 1038)	69.6 % (384 of 552)	81.3 % (326 of 401)	90.6 % (77 of 85)
Backbone	94.2 % (473 of 502)	93.7 % (163 of 174)	93.2 % (234 of 251)	98.7 % (76 of 77)
Sidechain	63.3 % (388 of 613)	58.5 % (221 of 378)	73.1 % (166 of 227)	12.5 % (1 of 8)
Aromatic	10.3 % (7 of 68)	20.6 % (7 of 34)	0.0 % (0 of 33)	0.0 % (0 of 1)
Methyl	86.3 % (69 of 80)	85.0 % (34 of 40)	87.5 % (35 of 40)

1. PDEgamma

MNLEPPKAEI RSATRVMGGP VTPRKGPPKF KQRQTRQFKS KPPKKGVQGF GDDIPGMEGL GTDITVIAPW EAFNHLELHE LAQYGII

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PDEgamma	[U-100% 15N]		0.05 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	PDEgamma	[U-100% 13C; U-100% 15N]		0.09 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	PROXYL-PDEgamma	[U-15N]		0.05 mM

Sample #4

Pressure 1 atm, Temperature 298 (±0.1) K, pH 4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	PROXYL-PDEgamma	[U-100% 15N]		0.05 mM
5	Ascorbic Acid	natural abundance		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

Release date

2007-08-19

Citation

Intrinsically disordered gamma-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure
Song, J., Guo, L., Muradov, H., Artemyev, N.O., Ruoho, A.E., Markley, J.L.
Proc. Natl. Acad. Sci. U. S. A. (2008), 105, 1505-1510, PubMed 18230733 , DOI 10.1073/pnas.0709558105 , Abstract

Related entities 1. PDEgamma, : 1 : 10 : 9 entities Detail

Interaction partners 1. PDEgamma, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC