Solution-State Structures of Oleate-Liganded LFABP, Protein Only
MNFSGKYQVQ SQENFEPFMK AMGLPEDLIQ KGKDIKGVSE IVHEGKKVKL TITYGSKVIH NEFTLGEECE LETMTGEKVK AVVKMEGDNK MVTTFKGIKS VTEFNGDTIT NTMTLGDIVY KRVSKRI
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.4 % (1426 of 1511) | 91.9 % (726 of 790) | 96.8 % (567 of 586) | 98.5 % (133 of 135) |
Backbone | 98.9 % (750 of 758) | 98.9 % (261 of 264) | 99.2 % (366 of 369) | 98.4 % (123 of 125) |
Sidechain | 91.0 % (790 of 868) | 88.4 % (465 of 526) | 94.9 % (315 of 332) | 100.0 % (10 of 10) |
Aromatic | 82.6 % (76 of 92) | 84.8 % (39 of 46) | 80.4 % (37 of 46) | |
Methyl | 100.0 % (136 of 136) | 100.0 % (68 of 68) | 100.0 % (68 of 68) |
1. LFABP
MNFSGKYQVQ SQENFEPFMK AMGLPEDLIQ KGKDIKGVSE IVHEGKKVKL TITYGSKVIH NEFTLGEECE LETMTGEKVK AVVKMEGDNK MVTTFKGIKS VTEFNGDTIT NTMTLGDIVY KRVSKRISolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details U-13C, 15N-LFABP with 3 equivalents of unlabeled-oleate to LFABP, pH7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | LFABP | [U-99% 13C; U-99% 15N] | 0.7 ~ 1.0 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | EDTA | natural abundance | 5 uM | |
5 | D2O | natural abundance | 5 % | |
6 | oleate | natural abundance | 3 eq | |
7 | sodium chloride | natural abundance | 100 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details U-13C, 15N-LFABP with 3 equivalents of unlabeled oleate to LFABP, pD7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | LFABP | [U-99% 13C; U-99% 15N] | 0.7 ~ 1.0 mM | |
9 | sodium phosphate | natural abundance | 50 mM | |
10 | sodium azide | natural abundance | 0.02 % | |
11 | EDTA | natural abundance | 5 uM | |
12 | D2O | natural abundance | 98 % | |
13 | oleate | natural abundance | 3 eq | |
14 | sodium chloride | natural abundance | 100 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details U-N15-LFABP with 3 equivalents of U-13C-oleate, pH7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | LFABP | [U-99% 15N] | 0.5 ~ 1.3 mM | |
16 | sodium phosphate | natural abundance | 50 mM | |
17 | sodium azide | natural abundance | 0.02 % | |
18 | EDTA | natural abundance | 5 uM | |
19 | D2O | natural abundance | 5 % | |
20 | oleate | [U-99% 13C] | 3 eq | |
21 | sodium chloride | natural abundance | 100 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details N15-LFABP with 3 equivalents of U-13C-oleate in the C12/E5 alignment medium
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
22 | LFABP | [U-99% 15N] | 0.3 mM | |
23 | sodium phosphate | natural abundance | 50 mM | |
24 | sodium azide | natural abundance | 0.02 % | |
25 | EDTA | natural abundance | 5 uM | |
26 | D2O | natural abundance | 5 % | |
27 | C12E5 | natural abundance | 5 % | |
28 | oleate | [U-99% 13C] | 3 eq | |
29 | sodium chloride | natural abundance | 100 mM |