BMRB: 15440

1H, 13C and 15N resonance assignments of the C-terminal domain of HasB from Serratia marcescens

Authors

Lefevre, J., Simenel, C., Delepelaire, P., Delepierre, M., Izadi-Pruneyre, N.

Assembly

HasB

Entity

1. HasB (polymer, Thiol state: not present), 132 monomers, 14404.29 Da Detail

MKVQEQSVGA PPPGRADKTA APQTRLTPYA QAGEDNWRSR ISGRLNRFKR YPKDALRLKR QGVGQVRFTL DRQGHVLAVT LVSSAGLPSL DREIQALVKR ASPLPTPPAD AYVNGTVELT LPIDFSLRGA GF

Formula weight

14404.29 Da

Source organism

Serratia marcescens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 93.1 %, Completeness (bb): 94.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (1409 of 1514)	93.3 % (742 of 795)	92.3 % (542 of 587)	94.7 % (125 of 132)
Backbone	94.7 % (727 of 768)	95.8 % (252 of 263)	93.5 % (360 of 385)	95.8 % (115 of 120)
Sidechain	92.2 % (799 of 867)	92.1 % (490 of 532)	92.6 % (299 of 323)	83.3 % (10 of 12)
Aromatic	87.5 % (70 of 80)	95.0 % (38 of 40)	79.5 % (31 of 39)	100.0 % (1 of 1)
Methyl	98.7 % (150 of 152)	100.0 % (76 of 76)	97.4 % (74 of 76)

1. HasB

MKVQEQSVGA PPPGRADKTA APQTRLTPYA QAGEDNWRSR ISGRLNRFKR YPKDALRLKR QGVGQVRFTL DRQGHVLAVT LVSSAGLPSL DREIQALVKR ASPLPTPPAD AYVNGTVELT LPIDFSLRGA GF

Show sample condition

Sample

Solvent system 85% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	HasB	[U-99% 13C; U-99% 15N]		0.8 mM
2	Sodium Phosphate			50 mM
3	NaCl			50 mM

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2M2K, Strand ID: A Detail

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Release date

2007-08-27

Citation

(1)H, (13)C and (15)N resonance assignments of the C-terminal domain of HasB, a specific TonB like protein, from Serratia marcescens
Lefevre, J., Simenel, C., Delepelaire, P., Delepierre, M., Izadi-Pruneyre, N.
Biomol. NMR Assign. (2007), 1, 197-199, PubMed 19636864 , DOI 10.1007/s12104-007-9055-7 , Abstract

Related entities 1. HasB, : 1 : 1 entities Detail

Experiments performed 8 experiments Detail