BMRB: 15449

Type I rat fatty acid synthase acyl carrier protein (ACP) domain

Authors

Ploskon, E.A., Arthur, C.J., Evans, S.E., Williams, C., Crosby, J., Crump, M.P.

Assembly

ACP domain from rat

Entity

1. ACP domain from rat (polymer, Thiol state: not present), 89 monomers, 9685.965 Da Detail

GDGEAQRDLV KAVAHILGIR DLAGINLDSS LADLGLDSLM GVEVRQILER EHDLVLPIRE VRQLTLRKLQ EMSSKAGSDT ELAAPKSKN

Formula weight

9685.965 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.1 %, Completeness (bb): 84.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.1 % (882 of 1001)	92.7 % (484 of 522)	79.8 % (308 of 386)	96.8 % (90 of 93)
Backbone	84.0 % (445 of 530)	91.8 % (168 of 183)	73.5 % (191 of 260)	98.9 % (86 of 87)
Sidechain	93.7 % (518 of 553)	93.2 % (316 of 339)	95.2 % (198 of 208)	66.7 % (4 of 6)
Aromatic	0.0 % (0 of 8)	0.0 % (0 of 4)	0.0 % (0 of 4)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. Fatty acid synthase (EC 2.3.1.85)

GDGEAQRDLV KAVAHILGIR DLAGINLDSS LADLGLDSLM GVEVRQILER EHDLVLPIRE VRQLTLRKLQ EMSSKAGSDT ELAAPKSKN

Show sample condition

Sample

Solvent system 20MM SODIUM DEUTEROACETATE, 1MM NAN3, 1MMDTT, PH 5.5, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 1-2MM ACP; 20MM SODIUM DEUTEROACETATE, 1MM NAN3, 1MMDTT, PH 5.5

#	Name	Isotope labeling	Type	Concentration
1	ACP			1.0 ~ 2.0 mM
2	sodium deuteroacetate	natural abundance		20 mM
3	sodium azide	natural abundance		1 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]		10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 30 models in PDB: 2PNG, Strand ID: A Detail

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Release date

2008-01-27

Citation

A mammalian type I fatty acid synthase acyl carrier protein domain does not sequester acyl chains
Ploskon, E.A., Arthur, C.J., Evans, S.E., Williams, C., Crosby, J., Simpson, T.J., Crump, M.P.
J. Biol. Chem. (2008), 283, 518-528, PubMed 17971456 , DOI 10.1074/jbc.M703454200 , Abstract

Related entities 1. ACP domain from rat, : 1 : 2 : 17 entities Detail

Interaction partners 1. ACP domain from rat, : 4 interactors Detail

Experiments performed 1 experiments Detail