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BMRB: 15490

2JVV

Solution Structure of E coli NusG carboxyterminal domain

Authors

Schweimer, K., Scheckenhofer, U., Roesch, P.

Assembly

NusG

Entity

1. NusG (polymer, Thiol state: all free), 181 monomers, 20531.26 Da Detail

MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV EIRGGQRRKS ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP APISDKEVDA IMNRLQQVGD KPRPKTLFEP GEMVRVNDGP FADFNGVVEE VDYEKSRLKV SVSIFGRATP VELDFSQVEK A

Formula weight

20531.26 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 40.9 %, Completeness: 35.2 %, Completeness (bb): 37.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	35.2 % (752 of 2138)	35.3 % (396 of 1121)	34.7 % (289 of 834)	36.6 % (67 of 183)
Backbone	37.7 % (401 of 1064)	38.9 % (142 of 365)	36.3 % (192 of 529)	39.4 % (67 of 170)
Sidechain	33.4 % (414 of 1241)	33.6 % (254 of 756)	33.9 % (160 of 472)	0.0 % (0 of 13)
Aromatic	30.0 % (51 of 170)	32.9 % (28 of 85)	27.7 % (23 of 83)	0.0 % (0 of 2)
Methyl	39.1 % (72 of 184)	41.3 % (38 of 92)	37.0 % (34 of 92)

1. NusG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	NusG	[U-95% 13C; U-95% 15N]	0.45 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.46 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JVV, Strand ID: A Detail

Release date

2009-06-09

Citation

Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators
Mooney, R., Schweimer, K., Roesch, P., Gottesman, M., Landick, R.
J. Mol. Biol. (2009), 391, 341-358, PubMed 19500594 , DOI 10.1016/j.jmb.2009.05.078 , Abstract

Related entities 1. NusG, : 1 : 47 : 2 : 1 : 65 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC