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BMRB: 15491

2JVW

Solution NMR structure of uncharacterized protein Q5E7H1 from Vibrio fischeri. Northeast Structural Genomics target VfR117.

Authors

Aramini, J.M., Wang, D., Nwosu, C., Owens, L.A., Xiao, R., Liu, J., Baran, M.C., Swapna, G.VT., Acton, T.B., Rost, B., Montelione, G.T.

Assembly

VfR117

Entity

1. VfR117 (polymer, Thiol state: all free), 88 monomers, 10724.22 Da Detail

MALIMTQQNN PLHGITLQKL LTELVEHYGW EELSYMVNIN CFKKDPSIKS SLKFLRKTDW ARERVENIYL KLQRHKERNQ LEHHHHHH

Formula weight

10724.22 Da

Source organism

Aliivibrio fischeri

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 90.1 %, Completeness (bb): 91.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1008 of 1119)	89.8 % (530 of 590)	90.0 % (387 of 430)	91.9 % (91 of 99)
Backbone	91.0 % (477 of 524)	90.3 % (159 of 176)	91.6 % (240 of 262)	90.7 % (78 of 86)
Sidechain	89.6 % (610 of 681)	89.6 % (371 of 414)	89.0 % (226 of 254)	100.0 % (13 of 13)
Aromatic	65.4 % (68 of 104)	65.4 % (34 of 52)	64.0 % (32 of 50)	100.0 % (2 of 2)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. VfR117

MALIMTQQNN PLHGITLQKL LTELVEHYGW EELSYMVNIN CFKKDPSIKS SLKFLRKTDW ARERVENIYL KLQRHKERNQ LEHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	VfR117	[U-100% 13C; U-100% 15N]	0.95 mM
2	ammonium acetate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
7	VfR117	[U-5% 13C; U-100% 15N]	1.08 mM
8	ammonium acetate	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JVW, Strand ID: A Detail

Release date

2007-09-25

Citation

Solution NMR structure of uncharacterized protein Q5E7H1 from Vibrio fischeri. Northeast Structural Genomics target VfR117
Aramini, J.M., Wang, D., Nwosu, C., Owens, L.A., Xiao, R., Liu, J., Baran, M.C., Swapna, G.VT., Acton, T.B., Rost, B., Montelione, G.T.

Related entities 1. VfR117, : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC