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BMRB: 15511

2JW8

1H,13C and 15N chemical shift assignments for stereo-array isotope labelled (SAIL) C-terminal dimerization domain of SARS coronavirus nucleocapsid protein

Authors

Takeda, M., Chang, C., Ikeya, T., Guntert, P., Chang, Y., Hsu, Y., Huang, T., Kainosho, M.

Assembly

C-terminal dimerization domain of SARS coronavirus nucleocapsid protein

Entity

1. C-terminal dimerization domain of SARS coronavirus nucleocapsid protein (polymer, Thiol state: not present), 128 monomers, 14532.18 × 2 Da Detail

MHHHHHHAMG TKKSAAEASK KPRQKRTATK QYNVTQAFGR RGPEQTQGNF GDQDLIRQGT DYKHWPQIAQ FAPSASAFFG MSRIGMEVTP SGTWLTYHGA IKLDDKDPQF KDNVILLNKH IDAYKTFP

Total weight

29064.36 Da

Max. entity weight

14532.18 Da

Source organism

SARS coronavirus AS

Exptl. method

solution NMR

Refine. method

simulated annealing, TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 66.4 %, Completeness (bb): 75.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.4 % (1008 of 1518)	64.8 % (516 of 796)	63.2 % (370 of 585)	89.1 % (122 of 137)
Backbone	75.6 % (570 of 754)	86.5 % (224 of 259)	63.1 % (236 of 374)	90.9 % (110 of 121)
Sidechain	61.5 % (542 of 882)	54.4 % (292 of 537)	72.3 % (238 of 329)	75.0 % (12 of 16)
Aromatic	42.6 % (69 of 162)	46.9 % (38 of 81)	36.7 % (29 of 79)	100.0 % (2 of 2)
Methyl	81.0 % (81 of 100)	82.0 % (41 of 50)	80.0 % (40 of 50)

1. SARS coronavirus nucleocapsid protein residues 248-365

MHHHHHHAMG TKKSAAEASK KPRQKRTATK QYNVTQAFGR RGPEQTQGNF GDQDLIRQGT DYKHWPQIAQ FAPSASAFFG MSRIGMEVTP SGTWLTYHGA IKLDDKDPQF KDNVILLNKH IDAYKTFP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	SARS coronavirus nucleocapsid protein residues 248-365	full SAIL isotope labeling	0.5 (±0.1) mM
2	D2O		10 %
3	H2O	natural abundance	90 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JW8, Strand ID: A, B Detail

Release date

2007-10-07

Citation

Solution structure of the c-terminal dimerization domain of SARS coronavirus nucleocapsid protein solved by the SAIL-NMR method
Takeda, M., Chang, C., Ikeya, T., Guntert, P., Chang, Y., Hsu, Y., Huang, T., Kainosho, M.
J. Mol. Biol. (2008), 380, 608-622, PubMed 18561946 , DOI 10.1016/j.jmb.2007.11.093 , Abstract

Related entities 1. C-terminal dimerization domain of SARS coronavirus nucleocapsid protein, : 1 : 1 : 1 : 73 entities Detail

Interaction partners 1. C-terminal dimerization domain of SARS coronavirus nucleocapsid protein, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC