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BMRB: 15524

2KWH

1H and 15N Resonance assignments for the human RLIP76 Ral binding domain

Authors

Fenwick, R.B., Prasannan, S., Campbell, L.J., Evetts, K.A., Nietlispach, D., Owen, D., Mott, H.R.

Assembly

RLIP76

Entity

1. RLIP76 (polymer, Thiol state: not present), 56 monomers, 6587.461 Da Detail

GSETQAGIKE EIRRQEFLLN SLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREA

Formula weight

6587.461 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 95.8 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	95.8 % (413 of 431)	95.7 % (353 of 369)	96.8 % (60 of 62)
Backbone	95.9 % (165 of 172)	95.7 % (111 of 116)	96.4 % (54 of 56)
Sidechain	95.8 % (248 of 259)	95.7 % (242 of 253)	100.0 % (6 of 6)
Aromatic	92.9 % (13 of 14)	92.3 % (12 of 13)	100.0 % (1 of 1)
Methyl	100.0 % (33 of 33)	100.0 % (33 of 33)

1. RLIP76

GSETQAGIKE EIRRQEFLLN SLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

Protein Blocks Logo

Calculated from 50 models in PDB: 2KWH, Strand ID: A Detail

Release date

2008-10-13

Citation

Resonance assignments for the RLIP76 Ral binding domain in its free form and in complex with the small G protein RalB
Fenwick, R., Prasannan, S., Campbell, L.J., Evetts, K.A., Nietlispach, D., Owen, D., Mott, H.R.
Biomol. NMR Assign. (2008), 2, 191-194, PubMed 19636902 , DOI 10.1007/s12104-008-9118-4 , Abstract

Related entities 1. RLIP76, : 1 : 1 : 9 : 9 entities Detail

Interaction partners 1. RLIP76, : 6 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

2 2D 1H-1H TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

3 2D DQF-COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

4 2D 1H-1H NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

5 3D 1H-15N NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

6 3D 1H-15N TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RLIP76	[U-98% 15N]		0.8 mM
2	D2O	[U-100% 2H]		10 %
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM
5	sodium azide	natural abundance		0.05 %
6	beta-mercaptoethanol	natural abundance		10 mM
7	magnesium chloride	natural abundance		1 mM

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