BMRB: 15553

1H, 13C and 15N resonance assignments from the Gal_lectin domain of mouse Latrophilin-1 GPCR

Authors

Vakonakis, I., Campbell, I.D.

Assembly

Gal lectin

Entity

1. Gal lectin (polymer, Thiol state: all disulfide bound), 106 monomers, 11987.55 Da Detail

GLPFGLMRRE LACEGYPIEL RCPGSDVIMV ENANYGRTDD KICDADPFQM ENVQCYLPDA FKIMSQRCNN RTQCVVVAGS DAFPDPCPGT YKYLEVQYDC VPYKVE

2. NAG (non-polymer), 221.208 Da

Total weight

12208.758 Da

Max. entity weight

11987.55 Da

Entity Connection

disulfide 4, covalent 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:ASN70:ND2	2:NAG1:C1
2	disulfide	sing	1:CYS13:SG	1:CYS43:SG
3	disulfide	sing	1:CYS22:SG	1:CYS100:SG
4	disulfide	sing	1:CYS55:SG	1:CYS87:SG
5	disulfide	sing	1:CYS68:SG	1:CYS74:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 96.2 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.2 % (1168 of 1214)	97.2 % (617 of 635)	94.5 % (446 of 472)	98.1 % (105 of 107)
Backbone	96.9 % (599 of 618)	98.6 % (207 of 210)	95.5 % (297 of 311)	97.9 % (95 of 97)
Sidechain	96.0 % (667 of 695)	96.5 % (410 of 425)	95.0 % (247 of 260)	100.0 % (10 of 10)
Aromatic	83.3 % (80 of 96)	85.4 % (41 of 48)	81.3 % (39 of 48)
Methyl	95.7 % (90 of 94)	95.7 % (45 of 47)	95.7 % (45 of 47)

1. Gal lectin

GLPFGLMRRE LACEGYPIEL RCPGSDVIMV ENANYGRTDD KICDADPFQM ENVQCYLPDA FKIMSQRCNN RTQCVVVAGS DAFPDPCPGT YKYLEVQYDC VPYKVE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Gal_lectin	[U-100% 15N]		1-2 (±0.1) mM
2	carbohydrate	[U-100% 15N]		1-2 (±0.1) mM
3	D2O	natural abundance		5 %
4	sodium azide	natural abundance		0.02 %
5	sodium phosphate	natural abundance		20 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)

#	Name	Isotope labeling	Type	Concentration
6	Gal_lectin	[U-100% 13C; U-100% 15N]		1-2 (±0.1) mM
7	carbohydrate	[U-100% 13C; U-100% 15N]		1-2 (±0.1) mM
8	D2O	natural abundance		5 %
9	sodium phosphate	natural abundance		20 mM
10	sodium azide	natural abundance		0.02 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)

#	Name	Isotope labeling	Type	Concentration
11	Gal_lectin	[U-100% 13C; U-100% 15N]		1-2 (±0.1) mM
12	carbohydrate	[U-100% 13C; U-100% 15N]		1-2 (±0.1) mM
13	D2O	natural abundance		100 %
14	sodium azide	natural abundance		0.02 %
15	sodium phosphate	natural abundance		20 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 2JX9, Strand ID: A Detail

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Release date

2008-04-20

Citation

Solution structure and sugar-binding mechanism of mouse latrophilin-1 RBL: a 7TM receptor-attached lectin-like domain
Vakonakis, I., Langenhan, T., Promel, S., Russ, A., Campbell, I.
Structure (2008), 16, 944-953, PubMed 18547526 , DOI 10.1016/j.str.2008.02.020 , Abstract

Related entities 1. Gal lectin, : 1 : 5 : 1 : 3 : 145 entities Detail

Interaction partners 1. Gal lectin, : 2 interactors Detail

Experiments performed 12 experiments Detail