BMRB: 15571

NMR STRUCTURE OF RNA DUPLEX

Authors

Popenda, L., Adamiak, R.W., Gdaniec, Z.

Assembly

RNA DUPLEX

Entity

1. RNA (5'-R(*GP*CP*AP*GP*AP*GP*AP*GP*CP*G)-3') (polymer, Thiol state: not present), 10 monomers, 3342.024 Da Detail

GCAGAGAGCG

2. RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3') (polymer, Thiol state: not present), 10 monomers, 3152.832 Da Detail

CGCUCUCUGC

Total weight

6494.856 Da

Max. entity weight

3342.024 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.8 %, Completeness (bb): 94.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	31P
All	94.8 % (313 of 330)	97.2 % (172 of 177)	93.2 % (124 of 133)	85.0 % (17 of 20)
Suger, PO4	94.2 % (226 of 240)	98.3 % (118 of 120)	91.0 % (91 of 100)	85.0 % (17 of 20)
Nucleobase	96.7 % (87 of 90)	94.7 % (54 of 57)	100.0 % (33 of 33)
Aromatic	97.4 % (74 of 76)	95.3 % (41 of 43)	100.0 % (33 of 33)

1. RNA (5'-R(*GP*CP*AP*GP*AP*GP*AP*GP*CP*G)-3')

GCAGAGAGCG

2. RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')

CGCUCUCUGC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*GP*CP*AP*GP*AP*GP*AP*GP*CP*G)-3')	natural abundance		0.5 mM
2	RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')	natural abundance		0.5 mM
3	D2O	[U-100% 2H]		100 %
4	NaCl	natural abundance		50 mM
5	sodium phosphate	natural abundance		10 mM
6	EDTA	natural abundance		0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
7	RNA (5'-R(*GP*CP*AP*GP*AP*GP*AP*GP*CP*G)-3')	natural abundance		0.5 mM
8	RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')	natural abundance		0.5 mM
9	D2O	[U-100% 2H]		10 %
10	H2O			90 %
11	NaCl	natural abundance		50 mM
12	sodium phosphate	natural abundance		10 mM
13	EDTA	natural abundance		0.1 mM

Hide sample codition

Release date

2008-05-28

Citation

Bulged adenosine influence on the RNA duplex conformation in solution
Popenda, L., Adamiak, R.W., Gdaniec, Z.
Biochemistry (2008), 47, 5059-5067, PubMed 18399645 , DOI 10.1021/bi7024904 , Abstract

Experiments performed 7 experiments Detail