BMRB: 15572

NMR STRUCTURE OF RNA DUPLEX CONTAINING SINGLE ADENOSINE BULGE

Authors

Popenda, L., Adamiak, R.W., Gdaniec, Z.

Assembly

RNA DUPLEX CONTAINING SINGLE ADENOSINE BULGE

Entity

1. RNA (5'-R(*GP*CP*AP*GP*AP*AP*GP*AP*GP*CP*G)-3') (polymer, Thiol state: not present), 11 monomers, 3671.230 Da Detail

GCAGAAGAGC G

2. RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3') (polymer, Thiol state: not present), 10 monomers, 3152.832 Da Detail

CGCUCUCUGC

Total weight

6824.062 Da

Max. entity weight

3671.23 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.9 %, Completeness (bb): 84.1 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	31P
All	87.9 % (304 of 346)	93.5 % (173 of 185)	80.0 % (112 of 140)	90.5 % (19 of 21)
Suger, PO4	84.1 % (212 of 252)	92.1 % (116 of 126)	73.3 % (77 of 105)	90.5 % (19 of 21)
Nucleobase	97.9 % (92 of 94)	96.6 % (57 of 59)	100.0 % (35 of 35)
Aromatic	97.5 % (78 of 80)	95.6 % (43 of 45)	100.0 % (35 of 35)

1. RNA (5'-R(*GP*CP*AP*GP*AP*AP*GP*AP*GP*CP*G)-3')

GCAGAAGAGC G

2. RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')

CGCUCUCUGC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*GP*CP*AP*GP*AP*AP*GP*AP*GP*CP*G)-3')	natural abundance		0.5 mM
2	RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')	natural abundance		0.5 mM
3	D2O	[U-100% 2H]		100 %
4	NaCl	natural abundance		50 mM
5	sodium phosphate	natural abundance		10 mM
6	EDTA	natural abundance		0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
7	RNA (5'-R(*GP*CP*AP*GP*AP*AP*GP*AP*GP*CP*G)-3')	natural abundance		0.5 mM
8	RNA (5'-R(*CP*GP*CP*UP*CP*UP*CP*UP*GP*C)-3')	natural abundance		0.5 mM
9	D2O	[U-100% 2H]		10 %
10	H2O			90 %
11	NaCl	natural abundance		50 mM
12	sodium phosphate	natural abundance		10 mM
13	EDTA	natural abundance		0.1 mM

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Release date

2008-05-28

Citation

Bulged adenosine influence on the RNA duplex conformation in solution
Popenda, L., Adamiak, R.W., Gdaniec, Z.
Biochemistry (2008), 47, 5059-5067, PubMed 18399645 , DOI 10.1021/bi7024904 , Abstract

Related entities 1. RNA (5'-R(*GP*CP*AP*GP*AP*AP*GP*AP*GP*CP*G)-3'), : 1 entities Detail

Experiments performed 8 experiments Detail