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BMRB: 15574

2JXU

NMR solution structure of KP-TerB, a tellurite resistance protein from Klebsiella pneumoniae

Authors

Sheng-Kuo, C., Yuan-Chao, L., Chinpan, C.

Assembly

KP-TerB

Entity

1. KP-TerB (polymer, Thiol state: all free), 159 monomers, 17752.94 Da Detail

MSFFDKVKGA LTSGREELTR QVGRYKNKKF MQGTVAVCAR IAVASDGVSS EEKQKMIGFL RSSEELKVFD TAEVIEFFNK LVTSFDFDLE IGKGETMKYI LALKDQPEAA QLALRVGIAV AKSDGNFDDD EKSAVREIAR SLGFDPAEFG LLEHHHHHH

Formula weight

17752.94 Da

Source organism

Klebsiella pneumoniae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 94.8 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.8 % (1753 of 1849)	94.3 % (904 of 959)	95.3 % (691 of 725)	95.8 % (158 of 165)
Backbone	95.9 % (911 of 950)	95.7 % (314 of 328)	96.1 % (447 of 465)	95.5 % (150 of 157)
Sidechain	94.0 % (983 of 1046)	93.5 % (590 of 631)	94.6 % (385 of 407)	100.0 % (8 of 8)
Aromatic	86.3 % (138 of 160)	86.3 % (69 of 80)	86.3 % (69 of 80)
Methyl	98.2 % (167 of 170)	96.5 % (82 of 85)	100.0 % (85 of 85)

1. KP-TerB

MSFFDKVKGA LTSGREELTR QVGRYKNKKF MQGTVAVCAR IAVASDGVSS EEKQKMIGFL RSSEELKVFD TAEVIEFFNK LVTSFDFDLE IGKGETMKYI LALKDQPEAA QLALRVGIAV AKSDGNFDDD EKSAVREIAR SLGFDPAEFG LLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	KP-TerB	[U-99% 13C; U-99% 15N]	1.0 ~ 2.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	1.0
¹H	DSS	methyl carbons	external	direct	1.0
¹⁵N	[15N] ammonium chloride	nitrogen	external	indirect	1.0

Referencing offset: 0.15 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	1.0
¹H	DSS	methyl carbons	external	direct	1.0
¹⁵N	[15N] ammonium chloride	nitrogen	external	indirect	1.0

Referencing offset: 0.15 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	1.0
¹H	DSS	methyl carbons	external	direct	1.0
¹⁵N	[15N] ammonium chloride	nitrogen	external	indirect	1.0

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	1.0
¹H	DSS	methyl carbons	external	direct	1.0
¹⁵N	[15N] ammonium chloride	nitrogen	external	indirect	1.0

Referencing offset: -0.2 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JXU, Strand ID: A Detail

Release date

2008-06-26

Citation

NMR solution structure of KP-TerB, a tellurite-resistance protein from Klebsiella pneumoniae
Chiang, S., Lou, Y., Chen, C.
Protein Sci. (2008), 17, 785-789, PubMed 18305192 , DOI 10.1110/ps.073389408 , Abstract