BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	82.5 % (804 of 975)	88.1 % (444 of 504)	73.2 % (281 of 384)	90.8 % (79 of 87)
Backbone	90.2 % (444 of 492)	91.3 % (157 of 172)	89.2 % (214 of 240)	91.3 % (73 of 80)
Sidechain	76.7 % (427 of 557)	86.4 % (287 of 332)	61.5 % (134 of 218)	85.7 % (6 of 7)
Aromatic	46.7 % (71 of 152)	81.6 % (62 of 76)	8.3 % (6 of 72)	75.0 % (3 of 4)
Methyl	83.9 % (47 of 56)	75.0 % (21 of 28)	92.9 % (26 of 28)

1. FBP21

DPSKGRWVEG ITSEGYHYYY DLISGASQWE KPEGFQGDLK KTAVKTVWVE GLSEDGFTYY YNTETGESRW EKPDDLEHHH HHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-99% 15N]	0.5 mM
5	D2O	[U-100% 2H]	10 %
6	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.754 ppm	internal	indirect	0.25144
¹H	water	protons	4.754 ppm	internal	direct	1.0
¹⁵N	water	protons	4.754 ppm	internal	indirect	0.10133

Referencing offset: -0.13 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.754 ppm	internal	indirect	0.25144
¹H	water	protons	4.754 ppm	internal	direct	1.0
¹⁵N	water	protons	4.754 ppm	internal	indirect	0.10133

Referencing offset: -0.13 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.754 ppm	internal	indirect	0.25144
¹H	water	protons	4.754 ppm	internal	direct	1.0
¹⁵N	water	protons	4.754 ppm	internal	indirect	0.10133

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.754 ppm	internal	indirect	0.25144
¹H	water	protons	4.754 ppm	internal	direct	1.0
¹⁵N	water	protons	4.754 ppm	internal	indirect	0.10133

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JXW, Strand ID: A Detail

Release date

2007-11-29

Citation

Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21)
Huang, X., Beullens, M., Zhang, J., Zhou, Y., Nicolaescu, E., Lesage, B., Hu, Q., Wu, J., Bollen, M., Shi, Y.
J. Biol. Chem. (2009), 284, 25375-25387, PubMed 19592703 , DOI 10.1074/jbc.M109.024828 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q9Y2W2 released on 2005-09-13
    Title WBP11_HUMAN Entity WW domain-binding protein 11
  Swiss-Prot: O75554 released on 2005-06-07
    Title WBP4_HUMAN Entity WW domain-binding protein 4

Related entities 1. FBP21 entity, : 1 : 4 : 69 entities Detail

Interaction partners 1. FBP21 entity, : 58 interactors Detail

More details for 58 interactors identified by 5 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-99% 15N]	0.5 mM
5	D2O	[U-100% 2H]	10 %
6	H2O		90 %

2 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

3 3D C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

4 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

5 3D 1H-13C NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

6 2D 1H-1H TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

7 2D 1H-1H NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

8 3D HCCH-TOCSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

9 3D HCCH-COSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

10 3D HBHA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

11 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

12 3D HNCO

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

13 3D HN(CO)CA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

14 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.0 mM
2	D2O	[U-100% 2H]	10 %
3	H2O		90 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_15575_2jxw.nef
Input source #2: Coordindates	2jxw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70-----
DPSKGRWVEGITSEGYHYYYDLISGASQWEKPEGFQGDLKKTAVKTVWVEGLSEDGFTYYYNTETGESRWEKPDD
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DPSKGRWVEGITSEGYHYYYDLISGASQWEKPEGFQGDLKKTAVKTVWVEGLSEDGFTYYYNTETGESRWEKPDD

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	75	0	0	100.0

Content subtype: combined_15575_2jxw.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	767		98.7 (chain: A, length: 75)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1865 (1)	noe	98.7 (chain: A, length: 75)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	56 (56)	.	50.7 (chain: A, length: 75)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 98.7%
- Total number of assignments
  - ¹H chemical shifts: 431
  - ¹³C chemical shifts: 259
  - ¹⁵N chemical shifts: 77
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	455	431	94.7
¹³C chemical shifts	344	259	75.3
¹⁵N chemical shifts	81	77	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	156	153	98.1
¹³C chemical shifts	150	142	94.7
¹⁵N chemical shifts	72	70	97.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	299	278	93.0
¹³C chemical shifts	194	117	60.3
¹⁵N chemical shifts	9	7	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	18	69.2
¹³C chemical shifts	26	22	84.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	62	96.9
¹³C chemical shifts	60	0	0.0
¹⁵N chemical shifts	4	3	75.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 98.7%
- Total number of restraints: 1863
- Weight of restraints: 1.0 (1863)
- Potential type of restraints: square-well-parabolic (1863)
- Range of distance target values: 2.4, 4.4 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 50.7%
- Total number of restraints: 56
- Total number of restraints per polymer type: 56
- Weight of restraints: 1.0 (56)
- Potential type of restraints: square-well-parabolic (56)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords FBP21, WBP4, WW domain

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.463 seconds)

BMRB: 15575

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. FBP21 entity, : 1 : 4 : 69 entities Detail

Interaction partners 1. FBP21 entity, : 58 interactors Detail

Experiments performed 14 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 4 contents Detail