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BMRB: 15592

2JY8

NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1) in complex with ubiquitin. RDC refined

Authors

Long, J.E., Layfield, R., Searle, M.S.

Assembly

P62 UBA in complex with ubiquitin

Entity

1. P62 UBA (polymer, Thiol state: not present), 52 monomers, 5739.363 Da Detail

GSPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KH

2. ubiquitin (polymer, Thiol state: not present), 76 monomers, 8556.668 Da Detail

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Total weight

14296.031 Da

Max. entity weight

8556.668 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 44.5 %, Completeness: 39.5 %, Completeness (bb): 42.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.5 % (591 of 1495)	37.9 % (296 of 782)	41.4 % (239 of 577)	41.2 % (56 of 136)
Backbone	42.1 % (319 of 758)	41.6 % (109 of 262)	42.6 % (159 of 373)	41.5 % (51 of 123)
Sidechain	37.7 % (322 of 854)	36.0 % (187 of 520)	40.5 % (130 of 321)	38.5 % (5 of 13)
Aromatic	56.8 % (42 of 74)	56.8 % (21 of 37)	55.6 % (20 of 36)	100.0 % (1 of 1)
Methyl	35.1 % (52 of 148)	35.1 % (26 of 74)	35.1 % (26 of 74)

1. P62 UBA

GSPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KH

2. ubiquitin

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	1 mM
2	ubiquitin		6 mM
3	potassium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	50 mM
5	sodium azide	natural abundance	0.04 %
6	D2O	[U-100% 2H]	10 %
7	H2O		90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	entity	[U-100% 13C; U-100% 15N]	1 mM
9	ubiquitin		6 mM
10	potassium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	50 mM
12	sodium azide	natural abundance	0.04 %
13	D2O	[U-100% 2H]	10 %
14	H2O		90 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 5% Polyacrylamide gel

#	Name	Isotope labeling	Concentration
15	entity	[U-100% 15N]	1 mM
16	ubiquitin		6 mM
17	potassium phosphate	natural abundance	50 mM
18	sodium chloride	natural abundance	50 mM
19	sodium azide	natural abundance	0.04 %
20	D2O	[U-100% 2H]	10 %
21	H2O		90 %

Protein Blocks Logo

Calculated from 21 models in PDB: 2JY8, Strand ID: A Detail

RDC

45 RDC values in 1 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Release date

2008-01-15

Citation

Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch
Long, J.E., Gallagher, T.R., Cavey, J.R., Sheppard, P.W., Ralston, S.H., Layfield, R., Searle, M.S.
J. Biol. Chem. (2008), 283, 5427-5440, PubMed 18083707 , DOI 10.1074/jbc.M704973200 , Abstract

Related entities 1. P62 UBA, : 1 : 4 : 9 : 11 entities Detail

Related entities 2. ubiquitin, : 21 : 119 : 187 entities Detail

Interaction partners 1. P62 UBA, : 119 interactors Detail

More details for 119 interactors identified by 51 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC