BMRB: 15593

Solution structure of human protein C6orf130, a putative macro domain

Authors

Volkman, B.F., Lytle, B.L., Peterson, F.C.

Assembly

C6orf130

Entity

1. C6orf130 (polymer, Thiol state: all free), 160 monomers, 18015.47 Da Detail

GHHHHHHLEA SSLNEDPEGS RITYVKGDLF ACPKTDSLAH CISEDCRMGA GIAVLFKKKF GGVQELLNQQ KKSGEVAVLK RDGRYIYYLI TKKRASHKPT YENLQKSLEA MKSHCLKNGV TDLSMPRIGC GLDRLQWENV SAMIEEVFEA TDIKITVYTL

Formula weight

18015.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 91.3 %, Completeness (bb): 96.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (1706 of 1868)	89.8 % (874 of 973)	92.0 % (670 of 728)	97.0 % (162 of 167)
Backbone	96.4 % (918 of 952)	95.7 % (314 of 328)	96.8 % (453 of 468)	96.8 % (151 of 156)
Sidechain	87.6 % (932 of 1064)	86.8 % (560 of 645)	88.5 % (361 of 408)	100.0 % (11 of 11)
Aromatic	62.5 % (85 of 136)	77.9 % (53 of 68)	46.3 % (31 of 67)	100.0 % (1 of 1)
Methyl	98.8 % (170 of 172)	98.8 % (85 of 86)	98.8 % (85 of 86)

1. C6orf130

GHHHHHHLEA SSLNEDPEGS RITYVKGDLF ACPKTDSLAH CISEDCRMGA GIAVLFKKKF GGVQELLNQQ KKSGEVAVLK RDGRYIYYLI TKKRASHKPT YENLQKSLEA MKSHCLKNGV TDLSMPRIGC GLDRLQWENV SAMIEEVFEA TDIKITVYTL

Show sample condition

Sample

Solvent system 93% H2O, 7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.55 mM C6orf130 U-15N/13C, 20 mM BisTris, 200 mM sodium chloride, 2 mM DTT

#	Name	Isotope labeling	Type	Concentration
1	C6orf130	[U-100% 13C; U-100% 15N]		0.55 mM
2	BisTris	natural abundance		20 mM
3	sodium chloride	natural abundance		200 mM
4	DTT	natural abundance		2 mM
5	D2O	[U-100% 2H]		7 %
6	H2O			93 %

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LACS Plot; CA

Referencing offset: -0.47 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.47 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LGR, Strand ID: A Detail

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Release date

2008-01-22

Related entities 1. C6orf130, : 1 : 7 : 20 entities Detail

Interaction partners 1. C6orf130, : 2 interactors Detail

Experiments performed 3 experiments Detail