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BMRB: 15607

2JZ4

Putative 32 kDa myrosinase binding protein At3g16450.1 from Arabidopsis

Authors

Takeda, M., Sugimori, N., Torizawa, T., Terauchi, T., Ono, A.M., Yagi, H., Yamaguchi, Y., Kato, K., Ikeya, T., Guntert, P., Aceti, D.J., Markley, J.L., Kainosho, M.

Assembly

At3g16450

Entity

1. At3g16450 (polymer, Thiol state: not present), 299 monomers, 31891.05 Da Detail

AQKVEAGGGA GGASWDDGVH DGVRKVHVGQ GQDGVSSINV VYAKDSQDVE GGEHGKKTLL GFETFEVDAD DYIVAVQVTY DNVFGQDSDI ITSITFNTFK GKTSPPYGLE TQKKFVLKDK NGGKLVGFHG RAGEALYALG AYFATTTTPV TPAKKLSAIG GDEGTAWDDG AYDGVKKVYV GQGQDGISAV KFEYNKGAEN IVGGEHGKPT LLGFEEFEID YPSEYITAVE GTYDKIFGSD GLIITMLRFK TNKQTSAPFG LEAGTAFELK EEGHKIVGFH GKASELLHQF GVHVMPLTN

Formula weight

31891.05 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.7 %, Completeness: 72.7 %, Completeness (bb): 81.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.7 % (2422 of 3332)	73.3 % (1251 of 1707)	66.2 % (869 of 1313)	96.8 % (302 of 312)
Backbone	81.9 % (1456 of 1778)	92.6 % (588 of 635)	68.2 % (581 of 852)	98.6 % (287 of 291)
Sidechain	66.8 % (1208 of 1808)	61.8 % (663 of 1072)	74.1 % (530 of 715)	71.4 % (15 of 21)
Aromatic	39.3 % (128 of 326)	48.5 % (79 of 163)	29.8 % (48 of 161)	50.0 % (1 of 2)
Methyl	76.4 % (243 of 318)	78.0 % (124 of 159)	74.8 % (119 of 159)

1. At3g16450.1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 300.65 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	At3g16450.1	stereo-array isotope labeling (SAIL)	0.2 mM
2	potassium chloride	natural abundance	100 mM
3	bis-TRIS-d19	[U-99% 2H]	20 mM
4	D2O	[U-100% 2H]	10 %
5	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 7 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 7 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JZ4, Strand ID: A Detail

Release date

2008-11-24

Citation 1

Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR
Takeda, M., Sugimori, N., Torizawa, T., Terauchi, T., Ono, A.M., Yagi, H., Yamaguchi, Y., Kato, K., Ikeya, T., Jee, J., Guntert, P., Aceti, D.J., Markley, J.L., Kainosho, M.
FEBS J. (2008), 275, 5873-5884, PubMed 19021763 , DOI 10.1111/j.1742-4658.2008.06717.x , Abstract

Show more 1 citaion

Citation 2

(1)H, (13)C and (15)N backbone assignment of a 32 kDa hypothetical protein from Arabidopsis thaliana, At3g16450.1
Sugimori, N., Torizawa, T., Aceti, D.J., Thao, S., Markley, J.L., Kainosho, M.
J. Biomol. NMR (2004), 30, 357-358, PubMed 15756462 , DOI 10.1007/s10858-005-2324-9 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: O04311 released on 2014-10-01
Title JAL33_ARATH Entity Jacalin-related lectin 33

Related entities 1. At3g16450, : 1 : 2 : 155 entities Detail

Interaction partners 1. At3g16450, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC