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BMRB: 15608

2JZ5

NMR STRUCTURE OF PROTEIN VPA0419 FROM VIBRIO PARAHAEMOLYTICUS: NORTHEAST STRUCTURAL GENOMICS TARGET VPR68.

Authors

SINGARAPU, K., SUKUMARAN, D.K., ELETSKI, A., PARISH, D., Zhao, L., Jiang, M., Maglaqui, M., Xiao, R., Liu, J., Baran, M.C., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., SZYPERSKI, T.

Assembly

VPA0419

Entity

1. VPA0419 (polymer, Thiol state: all free), 91 monomers, 10284.44 Da Detail

AGEIGFIIKE GDEVADVTIF AETKDALESE LAKYIELAKS VCAGVEYNVS ELTEESKELT ARFKFEVSAE KLIFELKTRS LARLEHHHHH H

Formula weight

10284.44 Da

Source organism

Vibrio parahaemolyticus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 91.6 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.6 % (968 of 1057)	92.8 % (504 of 543)	89.6 % (378 of 422)	93.5 % (86 of 92)
Backbone	93.0 % (508 of 546)	93.0 % (173 of 186)	92.9 % (250 of 269)	93.4 % (85 of 91)
Sidechain	90.5 % (541 of 598)	92.7 % (331 of 357)	87.1 % (209 of 240)	100.0 % (1 of 1)
Aromatic	61.1 % (55 of 90)	77.8 % (35 of 45)	44.4 % (20 of 45)
Methyl	98.2 % (112 of 114)	96.5 % (55 of 57)	100.0 % (57 of 57)

1. VPA0419

AGEIGFIIKE GDEVADVTIF AETKDALESE LAKYIELAKS VCAGVEYNVS ELTEESKELT ARFKFEVSAE KLIFELKTRS LARLEHHHHH H

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.07 mM
2	NaCl		100 mM
3	D2O	[U-100% 2H]	10 %
4	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JZ5, Strand ID: A Detail

Release date

2008-02-12

Citation

NMR STRUCTURE OF PROTEIN VPA0419 FROM VIBRIO PARAHAEMOLYTICUS: NORTHEAST STRUCTURAL GENOMICS TARGET VPR68
SINGARAPU, K., SUKUMARAN, D.K., Xiao, R., Acton, T., Montelione, G.T., SZYPERSKI, T.

Related entities 1. VPA0419, : 1 : 5 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC