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BMRB: 15614

2JZB

Solution structure of the complex between E.coli NusA-AR2 and RNAP-aCTD

Authors

Prasch, S., Schweimer, K., Roesch, P.

Assembly

complex

Entity

1. actd (polymer, Thiol state: all free), 99 monomers, 11101.59 Da Detail

GPDLRDVRQP EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL TEIKDVLASR GLSLGMRLEN WPPASIADE

2. ar2 (polymer, Thiol state: all free), 74 monomers, 7817.621 Da Detail

GPSLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEA

Total weight

18919.21 Da

Max. entity weight

11101.59 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 86.3 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.3 % (1681 of 1947)	93.7 % (949 of 1013)	73.8 % (561 of 760)	98.3 % (171 of 174)
Backbone	82.5 % (840 of 1018)	95.4 % (333 of 349)	68.6 % (347 of 506)	98.2 % (160 of 163)
Sidechain	91.5 % (996 of 1089)	92.8 % (616 of 664)	89.1 % (369 of 414)	100.0 % (11 of 11)
Aromatic	68.2 % (45 of 66)	81.8 % (27 of 33)	51.6 % (16 of 31)	100.0 % (2 of 2)
Methyl	95.9 % (213 of 222)	95.5 % (106 of 111)	96.4 % (107 of 111)

1. actd

GPDLRDVRQP EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL TEIKDVLASR GLSLGMRLEN WPPASIADE

2. ar2

GPSLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	actd	[U-98% 13C; U-98% 15N]	0.7 mM
2	ar2	natural abundance	2.1 mM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	beta-mercaptoethanol	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
6	ar2	[U-98% 13C; U-98% 15N]	0.7 mM
7	actd	natural abundance	2.1 mM
8	potassium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	beta-mercaptoethanol	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JZB, Strand ID: A, B Detail

Release date

2009-11-02

Citation

structural basis of transcription elongation control: the NusA-aCTD complex
Prasch, S., Schweimer, K., Roesch, P.

Related entities 1. actd, : 1 : 57 : 90 entities Detail

Related entities 2. ar2, : 2 : 23 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC