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BMRB: 15617

2JZC

Northeast Structural Genomics Consortium Target YG1 (Alg13), Chemical Shift Assignments

Authors

Wang, X., Weldeghorghis, T., Zhang, G., Imepriali, B., Montelione, G.T., Prestegard, J.H.

Assembly

alg13

Entity

1. alg13 (polymer, Thiol state: all free), 224 monomers, 25066.36 Da Detail

MGSSHHHHHH SSGLVPRGSH MLEGIIEEKA LFVTCGATVP FPKLVSCVLS DEFCQELIQY GFVRLIIQFG RNYSSEFEHL VQERGGQRES QKIPIDQFGC GDTARQYVLM NGKLKVIGFD FSTKMQSIIR DYSDLVISHA GTGSILDSLR LNKPLIVCVN DSLMDNHQQQ IADKFVELGY VWSCAPTETG LIAGLRASQT EKLKPFPVSH NPSFERLLVE TIYS

Formula weight

25066.36 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 56.2 %, Completeness (bb): 75.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.2 % (1471 of 2616)	43.1 % (585 of 1356)	67.4 % (691 of 1025)	83.0 % (195 of 235)
Backbone	75.9 % (1007 of 1326)	57.1 % (261 of 457)	85.0 % (556 of 654)	88.4 % (190 of 215)
Sidechain	42.7 % (639 of 1496)	36.0 % (324 of 899)	53.7 % (310 of 577)	25.0 % (5 of 20)
Aromatic	15.6 % (35 of 224)	26.8 % (30 of 112)	3.6 % (4 of 111)	100.0 % (1 of 1)
Methyl	92.4 % (231 of 250)	92.8 % (116 of 125)	92.0 % (115 of 125)

1. Alg13

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 K, pH 6.7, Details Uniform 13C,15N,2H labeled

#	Name	Isotope labeling	Concentration
1	Alg13	[U-13C; U-15N; U-2H]	0.35 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 K, pH 6.7, Details 13C-labeled Leu, Val and Ile.cd1 methyl group

#	Name	Isotope labeling	Concentration
5	Alg13	[U-15N; U-2H; 13C,1H Leu, Val, Ile.D1 methyl]	0.35 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	100 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 K, pH 6.7, Details 13C,1H-labeled Leu, Val and Ile.cd1 methyl group and 1H-labeld Phe, the rest are 15N and 2H labeled

#	Name	Isotope labeling	Concentration
8	Alg13	[U-15N; U-2H; 13C,1H Leu, Val, Ile.D1 methyl; 1H-Phe]	0.35 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	100 mM

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 K, pH 6.7, Details Uniform 13C,15N,2H labeled

#	Name	Isotope labeling	Concentration
11	Alg13	[U-13C; U-15N; U-2H]	0.35 mM
12	sodium phosphate	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	sodium azide	natural abundance	0.02 %
15	acrylamide	natural abundance	3.5 w/v
16	(3-acrylamidopropyl)-trimethylammonium chloride	natural abundance	3.5 w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JZC, Strand ID: A Detail

RDC

115 RDC values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 297 K, pH 6.7 Detail

Release date

2008-01-23

Citation

Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase
Wang, X., Weldeghorghis, T., Zhang, G., Imepriali, B., Prestegard, J.H.
Structure (2008), 16, 965-975, PubMed 18547528 , DOI 10.1016/j.str.2008.03.010 , Abstract

Related entities 1. alg13, : 1 : 3 : 15 entities Detail

Interaction partners 1. alg13, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC