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BMRB: 15623

2K7D

1H, 13C, and 15N Chemical Shift Assignments for Ca2+-bound CaBP1

Authors

Ames, J.B.

Assembly

CaBP1

Entity

1. CaBP1 (polymer, Thiol state: all free), 167 monomers, 19429.80 Da Detail

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

2. CA (non-polymer), 40.078 Da

Total weight

19469.879 Da

Max. entity weight

19429.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 75.4 %, Completeness (bb): 90.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.4 % (1473 of 1953)	70.6 % (727 of 1030)	79.6 % (596 of 749)	86.2 % (150 of 174)
Backbone	90.3 % (898 of 994)	90.7 % (312 of 344)	89.5 % (436 of 487)	92.0 % (150 of 163)
Sidechain	64.1 % (713 of 1112)	60.5 % (415 of 686)	71.8 % (298 of 415)	0.0 % (0 of 11)
Aromatic	36.2 % (42 of 116)	56.9 % (33 of 58)	15.5 % (9 of 58)
Methyl	90.8 % (138 of 152)	90.8 % (69 of 76)	90.8 % (69 of 76)

1. CaBP1

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CaBP1	[U-99% 13C; U-99% 15N]	0.5 mM
2	CALCIUM ION		0.5 mM
3	potassium chloride		0.05 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2K7D, Strand ID: A Detail

Release date

2008-03-30

Citation

1H, 15N, and 13C chemical shift assignments of calcium-bound calcium-binding protein 1 (CaBP1)
Li, C., Ames, J.B.
Biomol. NMR Assign. (2008), 2, 61-63, PubMed 19636926 , DOI 10.1007/s12104-008-9085-9 , Abstract

Related entities 1. CaBP1, : 2 : 2 : 296 entities Detail

Interaction partners 1. CaBP1, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC