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BMRB: 15634

2K02

Solution Structure of Putative Ferrous Iron Transport Protein C (FeoC) of Klebsiella pneumoniae

Authors

Hung, K., Lin, Y., Chen, J., Huang, T.

Assembly

FeoC monomer

Entity

1. FeoC monomer (polymer, Thiol state: all free), 87 monomers, 9866.377 Da Detail

MASLMEVRDM LALQGRMEAK QLSARLQTPQ PLIDAMLERM EAMGKVVRIS ETSEGCLSGS CKSCPEGKAA CRQEWWALRL EHHHHHH

Formula weight

9866.377 Da

Source organism

Klebsiella pneumoniae

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 85.3 %, Completeness (bb): 92.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (850 of 997)	84.7 % (444 of 524)	85.6 % (327 of 382)	86.8 % (79 of 91)
Backbone	92.2 % (476 of 516)	92.6 % (163 of 176)	91.4 % (234 of 256)	94.0 % (79 of 84)
Sidechain	79.8 % (449 of 563)	80.7 % (281 of 348)	80.8 % (168 of 208)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 48)	0.0 % (0 of 24)	0.0 % (0 of 22)	0.0 % (0 of 2)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. FeoC

MASLMEVRDM LALQGRMEAK QLSARLQTPQ PLIDAMLERM EAMGKVVRIS ETSEGCLSGS CKSCPEGKAA CRQEWWALRL EHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	FeoC	[U-13C; U-15N]	0.7 mM
2	TRIS	natural abundance	20 mM
3	NaCl	natural abundance	50 mM
4	DTT	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K02, Strand ID: A Detail

Release date

2012-07-18

Citation

NMR structure note: the ferrous iron transport protein C (FeoC) from Klebsiella pneumoniae
Hung, K., Juan, T., Hsu, Y., Huang, T.
J. Biomol. NMR (2012), 53, 161-165, PubMed 22580893 , DOI 10.1007/s10858-012-9633-6 , Abstract

Related entities 1. FeoC monomer, : 1 : 3 : 22 entities Detail

Experiments performed 10 experiments Detail

1 3D HNCA