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BMRB: 15644

2K0A

1H, 15N and 13C chemical shift assignments for Rds3 protein

Authors

Loening, N., van Roon, A., Yang, J., Nagai, K., Neuhaus, D.

Assembly

Rds3

Entity

1. Rds3p (polymer, Thiol state: free and other bound), 109 monomers, 12330.37 Da Detail

GGSSRHQFDL IMCLKQPGVQ TGLLCEKCDG KCPICDSYVR PKRKVRVCEN CSFGKQAKNC IICNLNVGVN DAFYCWECCR LGKDKDGCPR ILNLGSNRLD RHFEKKKKV

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

12526.597 Da

Max. entity weight

12330.37 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS88:SG	2:ZN1:ZN
3	na	sing	1:CYS48:SG	2:ZN1:ZN
4	na	sing	1:CYS51:SG	2:ZN1:ZN
5	na	sing	1:CYS25:SG	2:ZN1:ZN
6	na	sing	1:CYS28:SG	2:ZN1:ZN
7	na	sing	1:CYS60:SG	2:ZN1:ZN
8	na	sing	1:CYS63:SG	2:ZN1:ZN
9	na	sing	1:CYS32:SG	2:ZN1:ZN
10	na	sing	1:CYS35:SG	2:ZN1:ZN
11	na	sing	1:CYS75:SG	2:ZN1:ZN
12	na	sing	1:CYS78:SG	2:ZN1:ZN

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.1 % (1246 of 1283)	97.8 % (667 of 682)	96.9 % (469 of 484)	94.0 % (110 of 117)
Backbone	96.0 % (620 of 646)	96.9 % (217 of 224)	96.2 % (305 of 317)	93.3 % (98 of 105)
Sidechain	98.5 % (725 of 736)	98.3 % (450 of 458)	98.9 % (263 of 266)	100.0 % (12 of 12)
Aromatic	94.7 % (72 of 76)	97.4 % (37 of 38)	91.9 % (34 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. Rds3p

GGSSRHQFDL IMCLKQPGVQ TGLLCEKCDG KCPICDSYVR PKRKVRVCEN CSFGKQAKNC IICNLNVGVN DAFYCWECCR LGKDKDGCPR ILNLGSNRLD RHFEKKKKV

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rds3p	[U-98% 15N]	350 uM
2	Tris buffer	[U-99% 2H]	20 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	[U-99% 2H]	1 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Rds3p	[U-98% 13C; U-98% 15N]	700 uM
6	Tris buffer	[U-99% 2H]	20 mM
7	sodium chloride	natural abundance	200 mM
8	DTT	[U-99% 2H]	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 2.42 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K0A, Strand ID: A Detail

Release date

2008-07-27

Citation

Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif
van Roon, A.M., Loening, N.M., Obayashi, E., Yang, J., Newman, A.J., Hernandez, H., Nagai, K., Neuhaus, D.
Proc. Natl. Acad. Sci. U. S. A. (2008), 105, 9621-9626, PubMed 18621724 , DOI 10.1073/pnas.0802494105 , Abstract

Related entities 1. Rds3p, : 1 : 18 : 73 entities Detail

Interaction partners 1. Rds3p, : 6 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC