BMRBj - BMREntryMaster

Found 1 Document (1.023 seconds)

BMRB: 15660

2M1S

NMR assignment of the arenaviral protein Z from Lassa fever virus

Authors

Volpon, L., Osborne, M.J., Borden, K.L.B.

Assembly

LFV-Z

Entity

1. LFV-Z (polymer, Thiol state: all free), 99 monomers, 10675.33 Da Detail

MGNKQAKAPE SKDSPRASLI PDATHLGPQF CKSCWFENKG LVECNNHYLC LNCLTLLLSV SNRCPICKMP LPTKLRPSAA PTAPPTGAAD SIRPPPYSP

2. ZN (non-polymer), 65.409 Da

Total weight

10740.739 Da

Max. entity weight

10675.33 Da

Source organism

Lassa mammarenavirus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 79.3 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.3 % (893 of 1126)	74.9 % (445 of 594)	82.9 % (364 of 439)	90.3 % (84 of 93)
Backbone	87.6 % (494 of 564)	89.8 % (168 of 187)	85.0 % (249 of 293)	91.7 % (77 of 84)
Sidechain	73.7 % (484 of 657)	68.1 % (277 of 407)	83.0 % (200 of 241)	77.8 % (7 of 9)
Aromatic	64.3 % (36 of 56)	64.3 % (18 of 28)	63.0 % (17 of 27)	100.0 % (1 of 1)
Methyl	95.7 % (88 of 92)	97.8 % (45 of 46)	93.5 % (43 of 46)

1. LFV-Z

MGNKQAKAPE SKDSPRASLI PDATHLGPQF CKSCWFENKG LVECNNHYLC LNCLTLLLSV SNRCPICKMP LPTKLRPSAA PTAPPTGAAD SIRPPPYSP

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	LFV-Z	[U-100% 15N]	0.2 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	sodium azide	natural abundance	3 mM
5	zinc sulfate	natural abundance	0.05 mM
6	TCEP	natural abundance	0.05 mM
7	D2O		7 %
8	H2O	natural abundance	93 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
17	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
18	potassium phosphate	natural abundance	20 mM
19	sodium chloride	natural abundance	200 mM
20	sodium azide	natural abundance	3 mM
21	zinc sulfate	natural abundance	0.05 mM
22	TCEP	natural abundance	0.05 mM
23	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl protons	external	indirect	0.1013291
¹H	DSS	protons	external	direct	1.0
¹³N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl protons	external	indirect	0.1013291
¹H	DSS	protons	external	direct	1.0
¹³N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl protons	external	indirect	0.1013291
¹H	DSS	protons	external	direct	1.0
¹³N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl protons	external	indirect	0.1013291
¹H	DSS	protons	external	direct	1.0
¹³N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M1S, Strand ID: A Detail

Release date

2008-06-04

Citation 1

Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E
Volpon, L., Osborne, M.J., Capul, A.A., de la Torre, J.C., Borden, K.L.B.
Proc. Natl. Acad. Sci. U. S. A. (2010), 107, 5441-5446, PubMed 20212144 , DOI 10.1073/pnas.0909877107 , Abstract

Show more 1 citaion

Citation 2

NMR assignment of the arenaviral protein Z from Lassa fever virus
Volpon, L., Osborne, M.J., Borden, K.L.B.
Biomol. NMR Assign. (2008), 2, 81-84, PubMed 18958179 , DOI 10.1007/s12104-008-9090-z , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: O73557 released on 2005-12-06
Title Z_LASSJ Entity RING finger protein Z

Related entities 1. LFV-Z, : 1 : 1 : 29 entities Detail

Interaction partners 1. LFV-Z, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	LFV-Z	[U-100% 15N]	0.2 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	sodium azide	natural abundance	3 mM
5	zinc sulfate	natural abundance	0.05 mM
6	TCEP	natural abundance	0.05 mM
7	D2O		7 %
8	H2O	natural abundance	93 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
17	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
18	potassium phosphate	natural abundance	20 mM
19	sodium chloride	natural abundance	200 mM
20	sodium azide	natural abundance	3 mM
21	zinc sulfate	natural abundance	0.05 mM
22	TCEP	natural abundance	0.05 mM
23	D2O		100 %

3 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	LFV-Z	[U-100% 15N]	0.2 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	sodium azide	natural abundance	3 mM
5	zinc sulfate	natural abundance	0.05 mM
6	TCEP	natural abundance	0.05 mM
7	D2O		7 %
8	H2O	natural abundance	93 %

4 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	LFV-Z	[U-100% 15N]	0.2 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	sodium azide	natural abundance	3 mM
5	zinc sulfate	natural abundance	0.05 mM
6	TCEP	natural abundance	0.05 mM
7	D2O		7 %
8	H2O	natural abundance	93 %

5 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
17	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
18	potassium phosphate	natural abundance	20 mM
19	sodium chloride	natural abundance	200 mM
20	sodium azide	natural abundance	3 mM
21	zinc sulfate	natural abundance	0.05 mM
22	TCEP	natural abundance	0.05 mM
23	D2O		100 %

6 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

7 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
17	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
18	potassium phosphate	natural abundance	20 mM
19	sodium chloride	natural abundance	200 mM
20	sodium azide	natural abundance	3 mM
21	zinc sulfate	natural abundance	0.05 mM
22	TCEP	natural abundance	0.05 mM
23	D2O		100 %

9 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

10 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

11 2D (HB)CB(CGCD)HD

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

12 2D (HB)CB(CGCDCE)HE

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

13 3D C(CO)NH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
9	LFV-Z	[U-100% 13C; U-100% 15N]	0.2 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	sodium azide	natural abundance	3 mM
13	zinc sulfate	natural abundance	0.05 mM
14	TCEP	natural abundance	0.05 mM
15	D2O		7 %
16	H2O	natural abundance	93 %

nullKeywords LFV-Z, RING domain

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Found 1 Document (1.023 seconds)

BMRB: 15660

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. LFV-Z, : 1 : 1 : 29 entities Detail

Interaction partners 1. LFV-Z, : 1 interactors Detail

Experiments performed 13 experiments Detail

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