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BMRB: 15661

2K0Z

Solution NMR Structure of protein hp1203 from Helicobacter pylori 26695

Authors

Wu, B., Yee, A., Lemak, A., Cort, J., Semest, A., Kenney, M.A., Arrowsmith, C.H.

Assembly

hp1203

Entity

1. hp1203 (polymer, Thiol state: all free), 110 monomers, 12931.35 Da Detail

MLEDYAISLE EVNFNDFIVV DVRELDEYEE LHLPNATLIS VNDQEKLADF LSQHKDKKVL LHCRAGRRAL DAAKSMHELG YTPYYLEGNV YDFEKYGFRM VYDDTCDKKN

Formula weight

12931.35 Da

Source organism

Helicobacter pylori

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.1 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1260 of 1311)	98.1 % (667 of 680)	93.0 % (479 of 515)	98.3 % (114 of 116)
Backbone	98.3 % (645 of 656)	98.6 % (219 of 222)	98.2 % (320 of 326)	98.1 % (106 of 108)
Sidechain	94.6 % (720 of 761)	97.8 % (448 of 458)	89.5 % (264 of 295)	100.0 % (8 of 8)
Aromatic	73.8 % (96 of 130)	92.3 % (60 of 65)	55.4 % (36 of 65)
Methyl	99.1 % (115 of 116)	100.0 % (58 of 58)	98.3 % (57 of 58)

1. hp1203

MLEDYAISLE EVNFNDFIVV DVRELDEYEE LHLPNATLIS VNDQEKLADF LSQHKDKKVL LHCRAGRRAL DAAKSMHELG YTPYYLEGNV YDFEKYGFRM VYDDTCDKKN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	unknown function protein hp1203 from Helicobacter pylori 26695	[U-99% 13C; U-99% 15N]	0.5 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	sodium azide	natural abundance	0.01 %
5	benzamidine	natural abundance	10 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	unknown function protein hp1203 from Helicobacter pylori 26695	[U-7% 13C; U-99% 15N]	0.5 mM
9	TRIS	[U-100% 2H]	10 mM
10	sodium chloride	natural abundance	300 mM
11	sodium azide	natural abundance	0.01 %
12	benzamidine	natural abundance	10 mM
13	H2O		90 %
14	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K0Z, Strand ID: A Detail

Release date

2009-05-06

Citation

Solution NMR structure of protein hp1203 from helicobacter pyloric 26695/Northeast Structure Genomics Consortium (NESG) target PT1/Ontario Center for Structural Proteomics target hp1203
Wu, B., Yee, A., Lemak, A., Cort, J., Semest, A., Kenney, M.A., Arrowsmith, C.H.

Related entities 1. hp1203, : 1 : 17 entities Detail

Experiments performed 15 experiments Detail

1 3D HNCO