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BMRB: 15663

2LC5

Almost Complete Chemical Shift Assignments for a new calcium binding protein, EhCaM

Authors

Rout, A., Barnwal, R., Chary, K.

Assembly

CaBP3 monomer

Entity

1. Calcium Binding Protein 3 (polymer, Thiol state: not present), 151 monomers, 17236.08 Da Detail

MSEQKKVLTA EEQQEYKEAF QLFDKDNDNK LTAEELGTVM RALGANPTKQ KISEIVKDYD KDNSGKFDQE TFLTIMLEYG QEVDSTEDIK KAFEIFDKEK NGYISASELK HVLTTLGEKL TEQEVDDLLK EIGVEEGLIN VDDFVKLITS K

2. CA (non-polymer), 40.078 Da

Total weight

17276.158 Da

Max. entity weight

17236.08 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 72.8 %, Completeness (bb): 92.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.8 % (1309 of 1799)	64.4 % (605 of 940)	81.6 % (567 of 695)	83.5 % (137 of 164)
Backbone	92.8 % (839 of 904)	90.0 % (278 of 309)	95.3 % (424 of 445)	91.3 % (137 of 150)
Sidechain	58.5 % (607 of 1038)	51.8 % (327 of 631)	71.2 % (280 of 393)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 106)	0.0 % (0 of 53)	0.0 % (0 of 53)
Methyl	75.6 % (127 of 168)	76.2 % (64 of 84)	75.0 % (63 of 84)

1. Calcium Binding Protein 3

MSEQKKVLTA EEQQEYKEAF QLFDKDNDNK LTAEELGTVM RALGANPTKQ KISEIVKDYD KDNSGKFDQE TFLTIMLEYG QEVDSTEDIK KAFEIFDKEK NGYISASELK HVLTTLGEKL TEQEVDDLLK EIGVEEGLIN VDDFVKLITS K

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	EhCaM	[U-99% 15N]		1.1 mM
2	calcium2+	natural abundance		30 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	EhCaM	[U-95% 13C]		1.2 mM
4	calcium2+	natural abundance		30 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	EhCaM	[U-99% 13C; U-99% 15N]		1.1 mM
6	calcium2+	natural abundance		30 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.73 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.73 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.39 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LC5, Strand ID: A Detail

Release date

2008-04-13

Citation

Sequence specific 1H, 13C and 15N resonance assignments of a calmodulin-like calcium-binding protein from the protozoan parasite Entamoeba histolytica (EhCaM)
Rout, A.K., Barnwal, R.P., Padhan, N., Bhattacharya, A., Chary, K.V.R.
Biomol. NMR Assign. (2008), 2, 77-79, PubMed 19636930 , DOI 10.1007/s12104-008-9089-5 , Abstract

Related entities 1. Calcium Binding Protein 3, : 1 : 296 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC