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Found 1 Document (1.226 seconds)

BMRB: 15691

2K29

Structure of the DBD domain of E. coli antitoxin RelB

Authors

Li, G., Zhang, Y., Inouye, M., Ikura, M.

Assembly

RelB

Entity

1. RelB (polymer, Thiol state: not available), 53 monomers, 5993.844 × 2 Da Detail

GSHMGSINLR IDDELKARSY AALEKMGVTP SEALRLMLEY IADNERLPFK QTL

Total weight

11987.688 Da

Max. entity weight

5993.844 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 92.3 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	92.3 % (349 of 378)	91.7 % (297 of 324)	96.3 % (52 of 54)
Backbone	93.7 % (148 of 158)	92.5 % (99 of 107)	96.1 % (49 of 51)
Sidechain	91.4 % (201 of 220)	91.2 % (198 of 217)	100.0 % (3 of 3)
Aromatic	80.0 % (12 of 15)	80.0 % (12 of 15)
Methyl	90.3 % (28 of 31)	90.3 % (28 of 31)

1. RelB

GSHMGSINLR IDDELKARSY AALEKMGVTP SEALRLMLEY IADNERLPFK QTL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	DTT	natural abundance	1 mM
10	sodium azide	natural abundance	1 uM

Protein Blocks Logo

Calculated from 20 models in PDB: 2K29, Strand ID: A, B Detail

RDC

42 RDC values in 1 lists
Field strength (¹H) 600.1716 MHz, Pressure 1 atm, Temperature 303 K, pH 7.0 Detail

Release date

2008-06-26

Citation

Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module
Li, G., Zhang, Y., Inouye, M., Ikura, M.
J. Mol. Biol. (2008), 380, 107-119, PubMed 18501926 , DOI 10.1016/j.jmb.2008.04.039 , Abstract

Related entities 1. RelB, : 1 : 3 : 8 entities Detail

Interaction partners 1. RelB, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

2 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

4 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

5 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

6 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

7 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	DTT	natural abundance	1 mM
10	sodium azide	natural abundance	1 uM

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	DTT	natural abundance	1 mM
10	sodium azide	natural abundance	1 uM

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 uM

nullKeywords antitoxin, cooperative binding, RelB, RHH, transcriptional repressor

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Found 1 Document (1.226 seconds)

BMRB: 15691

Sample #1

Sample #2

Related entities 1. RelB, : 1 : 3 : 8 entities Detail

Interaction partners 1. RelB, : 2 interactors Detail

Experiments performed 10 experiments Detail

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