BMRB: 15691

Structure of the DBD domain of E. coli antitoxin RelB

Authors

Li, G., Zhang, Y., Inouye, M., Ikura, M.

Assembly

RelB

Entity

1. RelB (polymer, Thiol state: not available), 53 monomers, 5993.844 × 2 Da Detail

GSHMGSINLR IDDELKARSY AALEKMGVTP SEALRLMLEY IADNERLPFK QTL

Total weight

11987.688 Da

Max. entity weight

5993.844 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 92.3 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	92.3 % (349 of 378)	91.7 % (297 of 324)	96.3 % (52 of 54)
Backbone	93.7 % (148 of 158)	92.5 % (99 of 107)	96.1 % (49 of 51)
Sidechain	91.4 % (201 of 220)	91.2 % (198 of 217)	100.0 % (3 of 3)
Aromatic	80.0 % (12 of 15)	80.0 % (12 of 15)
Methyl	90.3 % (28 of 31)	90.3 % (28 of 31)

1. RelB

GSHMGSINLR IDDELKARSY AALEKMGVTP SEALRLMLEY IADNERLPFK QTL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	RelBN	[U-100% 13C; U-100% 15N]		0.5-1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	natural abundance		1 mM
5	sodium azide	natural abundance		1 uM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	RelBN	[U-100% 13C; U-100% 15N]		0.5-1.0 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		100 mM
9	DTT	natural abundance		1 mM
10	sodium azide	natural abundance		1 uM

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Calculated from 20 models in PDB: 2K29, Strand ID: A, B Detail

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RDC

42 RDC values in 1 lists
Field strength (¹H) 600.1716 MHz, Pressure 1 atm, Temperature 303 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 303 K, pH 7.0

Experiment name 2D 1H-15N HSQC

List #1 RDC_list_1, RDC code DNH, Field strength (¹H) 600.1716 MHz

Release date

2008-06-26

Citation

Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module
Li, G., Zhang, Y., Inouye, M., Ikura, M.
J. Mol. Biol. (2008), 380, 107-119, PubMed 18501926 , DOI 10.1016/j.jmb.2008.04.039 , Abstract

Related entities 1. RelB, : 1 : 3 : 8 entities Detail

Interaction partners 1. RelB, : 2 interactors Detail

Experiments performed 10 experiments Detail