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BMRB: 15698

2K2A

Solution Structure of the Apo C terminal domain of Lethocerus troponin C isoform F1

Authors

De Nicola, G.FELICE., Kelly, G., Bullard, B., McCormick, J.

Assembly

Lethocerus F1 troponin C isoform

Entity

1. Lethocerus F1 troponin C isoform (polymer, Thiol state: not present), 70 monomers, 7845.448 × 10 Da Detail

MQQELREAFR LYDKEGNGYI STDVMREILA ELDETLSSED LDAMIDEIDA DGSGTVDFEE FMGVMTGGDE

Total weight

78454.484 Da

Max. entity weight

7845.448 Da

Source organism

Lethocerus indicus

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.3 %, Completeness (bb): 80.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (644 of 764)	93.6 % (368 of 393)	68.8 % (205 of 298)	97.3 % (71 of 73)
Backbone	80.7 % (339 of 420)	95.9 % (141 of 147)	64.0 % (130 of 203)	97.1 % (68 of 70)
Sidechain	89.2 % (363 of 407)	92.3 % (227 of 246)	84.2 % (133 of 158)	100.0 % (3 of 3)
Aromatic	78.3 % (36 of 46)	100.0 % (23 of 23)	56.5 % (13 of 23)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. Lethocerus F1 troponin C isoform

MQQELREAFR LYDKEGNGYI STDVMREILA ELDETLSSED LDAMIDEIDA DGSGTVDFEE FMGVMTGGDE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	protein	[U-99% 15N]	0.4 mM
2	sodium chloride	natural abundance	100 mM
3	TRIS	natural abundance	20 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
4	protein	[U-99% 15N]	0.4 mM
5	sodium chloride	natural abundance	100 mM
6	TRIS	natural abundance	20 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	protein	[U-99% 13C; U-99% 15N]	0.4 mM
8	sodium chloride	natural abundance	100 mM
9	TRIS	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2K2A, Strand ID: A Detail

Release date

2010-01-31

Citation

Solution structure of the Apo C-terminal domain of the Lethocerus F1 troponin C isoform
De Nicola, G., Martin, S., Bullard, B., Pastore, A.
Biochemistry (2010), 49, 1719-1726, PubMed 20104876 , DOI 10.1021/bi902094w , Abstract

Related entities 1. Lethocerus F1 troponin C isoform, : 1 : 1 : 562 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC