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BMRB: 15700

2K2C

solution struture of human N-terminal domain of pirh2/Northeast Structure Genomics Consortium (NESG) target HT2A

Authors

Wu, B., Lemak, A., Sheng, Y., Karra, M., Srisailam, S., Sunnerhagen, M., Arrowsmith, C.

Assembly

human N-termal doamin of pirh2

Entity

1. human N-termal doamin of pirh2 (polymer, Thiol state: free and other bound), 137 monomers, 15878.01 Da Detail

MAATAREDGA TGEERGQRGC EHYDRGCLLK APCCDKLYTC RLCHDNNEDH QLDRFKVKEV QCINCEKIQH AQQTCEECST LFGEYYCDIC HLFDKDKKQY HCENCGICRI GPKEDFFHCL KCNLCLAMNL QGRHKCI

2. ZN (non-polymer), 65.409 × 6 Da

Total weight

16270.464 Da

Max. entity weight

15878.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 87.3 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.3 % (1381 of 1581)	87.9 % (731 of 832)	86.2 % (517 of 600)	89.3 % (133 of 149)
Backbone	91.6 % (749 of 818)	91.5 % (257 of 281)	91.5 % (368 of 402)	91.9 % (124 of 135)
Sidechain	84.2 % (750 of 891)	86.0 % (474 of 551)	81.9 % (267 of 326)	64.3 % (9 of 14)
Aromatic	47.5 % (58 of 122)	57.4 % (35 of 61)	37.7 % (23 of 61)
Methyl	88.0 % (88 of 100)	88.0 % (44 of 50)	88.0 % (44 of 50)

1. human N-termal doamin of pirh2

MAATAREDGA TGEERGQRGC EHYDRGCLLK APCCDKLYTC RLCHDNNEDH QLDRFKVKEV QCINCEKIQH AQQTCEECST LFGEYYCDIC HLFDKDKKQY HCENCGICRI GPKEDFFHCL KCNLCLAMNL QGRHKCI

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	human N-termal doamin of pirh2	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	potassium chloride	natural abundance	150 mM
4	Zncl2	natural abundance	10 uM
5	DTT	natural abundance	0.8 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	human N-termal doamin of pirh2	[U-99% 13C; U-99% 15N]	1 mM
7	sodium phosphate	natural abundance	50 mM
8	potassium chloride	natural abundance	150 mM
9	Zncl2	natural abundance	10 uM
10	DTT	natural abundance	0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2K2C, Strand ID: A Detail

Release date

2009-04-02

Citation

Molecular basis of Pirh2-mediated p53 ubiquitylation
Sheng, Y., Laister, R.C., Lemak, A., Wu, B., Tai, E., Duan, S., Lukin, J., Sunnerhagen, M., Srisailam, S., Karra, M., Benchimol, S., Arrowsmith, C.H.
Nat. Struct. Mol. Biol. (2008), 15, 1334-1342, PubMed 19043414 , DOI 10.1038/nsmb.1521 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 15701 released on 2008-04-06
    Title solution struture of human C-terminal domain of pirh2/Northeast Structural Genomics Consortium (NESG) target HT2C
  BMRB: 15333 released on 2007-07-29
    Title Resonance Assignment of the human Pirh2 RING domain; Northeast Structural Genomics Consortium Target HT2B
  Swiss-Prot: Q96PM5 released on 2003-02-28
    Title ZN363_HUMAN Entity RING finger and CHY zinc finger domain-containing protein 1

Related entities 1. human N-termal doamin of pirh2, : 1 : 2 : 46 entities Detail

Interaction partners 1. human N-termal doamin of pirh2, : 30 interactors Detail

More details for 30 interactors identified by 8 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC