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BMRB: 15704

2K2F

Ca2+-S100A1-RyRP12

Authors

Wright, N.T., Varney, K.M., Weber, D.J.

Assembly

S100A1 bound to calmodulin

Entity

1. S100A1 (polymer, Thiol state: not present), 12 monomers, 1465.781 × 2 Da Detail

KKAVWHKLLS KQ

2. calmodulin (polymer, Thiol state: all free), 93 monomers, 10428.52 × 2 Da Detail

GSELETAMET LINVFHAHSG KEGDKYKLSK KELKDLLQTE LSSFLDVQKD ADAVDKIMKE LDENGDGEVD FQEFVVLVAA LTVACNNFFW ENS

3. CA (non-polymer), 40.078 × 3 Da

Total weight

23908.836 Da

Max. entity weight

10428.52 Da

Source organism

Rattus norvegicus , Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 15.2 %, Completeness: 12.7 %, Completeness (bb): 10.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	12.7 % (97 of 761)	13.6 % (88 of 645)	7.8 % (9 of 116)
Backbone	10.6 % (34 of 320)	11.6 % (25 of 215)	8.6 % (9 of 105)
Sidechain	14.3 % (63 of 441)	14.7 % (63 of 430)	0.0 % (0 of 11)
Aromatic	7.4 % (4 of 54)	7.7 % (4 of 52)	0.0 % (0 of 2)
Methyl	11.7 % (7 of 60)	11.7 % (7 of 60)

1. S100A1

KKAVWHKLLS KQ

2. calmodulin

GSELETAMET LINVFHAHSG KEGDKYKLSK KELKDLLQTE LSSFLDVQKD ADAVDKIMKE LDENGDGEVD FQEFVVLVAA LTVACNNFFW ENS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details S100A1-RyRP12 solution for NMR

#	Name	Isotope labeling	Concentration
1	CALCIUM ION	natural abundance	10-15 mM
2	entity_1	[U-100% 13C; U-100% 15N]	1-3 mM
3	entity_2	natural abundance	2-6 mM
4	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2K2F, Strand ID: A, B, C, D Detail

Release date

2008-08-27

Citation

S100A1 and calmodulin compete for the same binding site on ryanodine receptor
Wright, N.T., Prosser, B.L., Varney, K.M., Zimmer, D.B., Schneider, M.F., Weber, D.J.
J. Biol. Chem. (2008), 283, 26676-26683, PubMed 18650434 , DOI 10.1074/jbc.M804432200 , Abstract

Entries sharing articles Swiss-Prot: 7 entries Detail

  Swiss-Prot: B0LPN4 released on 2012-02-22
    Title RYR2_RAT Entity Ryanodine receptor 2
  Swiss-Prot: Q92736 released on 2001-09-26
    Title RYR2_HUMAN Entity Ryanodine receptor 2
  Swiss-Prot: P35467 released on 1994-06-01
    Title S10A1_RAT Entity Protein S100-A1
  Swiss-Prot: P23297 released on 1991-11-01
    Title S10A1_HUMAN Entity Protein S100-A1
  Swiss-Prot: P21817 released on 1991-05-01
    Title RYR1_HUMAN Entity Ryanodine receptor 1
  Swiss-Prot: P62158 released on 1986-07-21
    Title CALM_HUMAN Entity Calmodulin
  Swiss-Prot: P62204 released on 1986-07-21
    Title CALM_MOUSE Entity Calmodulin

Related entities 1. S100A1, : 1 : 20 entities Detail

Related entities 2. calmodulin, : 1 : 84 entities Detail

Interaction partners 1. S100A1, : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC