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Found 1 Document (1.081 seconds)

BMRB: 15717

2LB9

SOLUTION STRUCTURE OF A CYANOBACTERIAL PHYTOCHROME GAF DOMAIN IN THE RED LIGHT-ABSORBING GROUND STATE

Authors

Cornilescu, C.C., Cornilescu, G., Ulijasz, A.T., Vierstra, R.D., Markley, J.L.

Assembly

CYANOBACTERIAL PHYTOCHROME GAF

Entity

1. CYANOBACTERIAL PHYTOCHROME GAF (polymer, Thiol state: not present), 208 monomers, 23772.74 Da Detail

MDTETWAAAA RPSRDALINR ITHQIRQSLE LDQILRATVE EVRAFLGTDR VKVYRFDPEG HGTVVAEARG GERLPSLLGL TFPAGDIPEE ARRLFRLAQV RVIVDVEAQS RSISQPESWG LSARVPLGEP LQRPVDPXHV HYLKSMGVAS SLVVPLMHHQ ELWGLLVSHH AEPRPYSQEE LQVVQLLADQ VSIAIAQAEL SLHHHHHH

Formula weight

23772.74 Da

Source organism

Thermosynechococcus elongatus BP-1

Exptl. method

solution NMR

Refine. method

molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.3 %, Completeness: 58.3 %, Completeness (bb): 73.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	58.3 % (1390 of 2384)	50.7 % (626 of 1235)	64.9 % (609 of 938)	73.5 % (155 of 211)
Backbone	73.4 % (892 of 1216)	68.4 % (282 of 412)	75.6 % (461 of 610)	76.8 % (149 of 194)
Sidechain	47.6 % (649 of 1364)	41.8 % (344 of 823)	57.1 % (299 of 524)	35.3 % (6 of 17)
Aromatic	36.5 % (57 of 156)	42.3 % (33 of 78)	30.7 % (23 of 75)	33.3 % (1 of 3)
Methyl	64.4 % (174 of 270)	64.4 % (87 of 135)	64.4 % (87 of 135)

1. SyB-Cph1(GAF)

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
5	SyB-Cph1(GAF)	natural abundance	2 mM
6	PHYCOCYANOBILIN	[U-15N]	2 mM
7	Tris-DCl	[U-2H]	10 mM
8	NaN3	natural abundance	0.03 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
9	SyB-Cph1(GAF)	natural abundance	1 mM
10	PHYCOCYANOBILIN	[U-13C]	1 mM
11	Tris-DCl	[U-2H]	10 mM
12	NaN3	natural abundance	0.03 %

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
13	SyB-Cph1(GAF)	[U-13C; U-15N]	1 mM
14	PHYCOCYANOBILIN	natural abundance	1 mM
15	Tris-DCl	[U-2H]	10 mM
16	NaN3	natural abundance	0.03 %

Sample #5

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
17	SyB-Cph1(GAF)	[U-13C; U-15N]	1 mM
18	PHYCOCYANOBILIN	natural abundance	1 mM
19	Tris-DCl	[U-2H]	10 mM
20	NaN3	natural abundance	0.03 %

Sample #6

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
21	SyB-Cph1(GAF)	natural abundance	0.5 mM
22	PHYCOCYANOBILIN	[U-13C]	0.5 mM
23	Tris-DCl	[U-2H]	10 mM
24	NaN3	natural abundance	0.03 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	na	direct	1.0
¹⁵N	water	protons	4.773 ppm	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	na	direct	1.0
¹⁵N	water	protons	4.773 ppm	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	na	direct	1.0
¹⁵N	water	protons	4.773 ppm	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	na	direct	1.0
¹⁵N	water	protons	4.773 ppm	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LB9, Strand ID: A Detail

Release date

2008-09-11

Citation

Solution structure of a cyanobacterial phytochrome GAF domain in the red-light-absorbing ground state
Cornilescu, G., Ulijasz, A.T., Cornilescu, C.C., Markley, J.L., Vierstra, R.D.
J. Mol. Biol. (2008), 383, 403-413, PubMed 18762196 , DOI 10.1016/j.jmb.2008.08.034 , Abstract

Related entities 1. CYANOBACTERIAL PHYTOCHROME GAF, : 1 : 5 : 69 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

2 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

4 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

5 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

7 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
1	SyB-Cph1(GAF)	[U-13C; U-15N]	1.7 mM
2	PHYCOCYANOBILIN	natural abundance	1.7 mM
3	Tris-DCl	[U-2H]	10 mM
4	NaN3	natural abundance	0.03 %

11 3D HNCO antiphase

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 306 K, pH 8.5

#	Name	Isotope labeling	Concentration
17	SyB-Cph1(GAF)	[U-13C; U-15N]	1 mM
18	PHYCOCYANOBILIN	natural abundance	1 mM
19	Tris-DCl	[U-2H]	10 mM
20	NaN3	natural abundance	0.03 %

12 3D HCA(CO)N antiphase

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 306 K, pH 8.5

#	Name	Isotope labeling	Concentration
17	SyB-Cph1(GAF)	[U-13C; U-15N]	1 mM
18	PHYCOCYANOBILIN	natural abundance	1 mM
19	Tris-DCl	[U-2H]	10 mM
20	NaN3	natural abundance	0.03 %

13 J modulated 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
21	SyB-Cph1(GAF)	natural abundance	0.5 mM
22	PHYCOCYANOBILIN	[U-13C]	0.5 mM
23	Tris-DCl	[U-2H]	10 mM
24	NaN3	natural abundance	0.03 %

14 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
5	SyB-Cph1(GAF)	natural abundance	2 mM
6	PHYCOCYANOBILIN	[U-15N]	2 mM
7	Tris-DCl	[U-2H]	10 mM
8	NaN3	natural abundance	0.03 %

15 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 8.5

#	Name	Isotope labeling	Concentration
9	SyB-Cph1(GAF)	natural abundance	1 mM
10	PHYCOCYANOBILIN	[U-13C]	1 mM
11	Tris-DCl	[U-2H]	10 mM
12	NaN3	natural abundance	0.03 %

nullKeywords bacteriophytochrome, cyanobacterial phytochrome, GAF DOMAIN, PCB, phycocyanobilin, phytochrome

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Found 1 Document (1.081 seconds)

BMRB: 15717

Sample #1

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Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CYANOBACTERIAL PHYTOCHROME GAF, : 1 : 5 : 69 entities Detail

Experiments performed 15 experiments Detail

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