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BMRB: 15718

2K2O

Solution Structure of the inner DysF domain of human myoferlin

Authors

Patel, P., Harris, R., Keep, N., Driscoll, P.

Assembly

myoferlin

Entity

1. myoferlin (polymer, Thiol state: all free), 123 monomers, 14173.26 Da Detail

IDPFTADAGH TEFTDEVYQN ESRYPGGDWK PAEDTYTDAN GDKAASPSEL TCPPGWEWED DAWSYDINRA VDEKGWEYGI TIPPDHKPKS WVAAEKMYHT HRRRRLVRKR KKDLTQTASS TAR

Formula weight

14173.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, conformer_family_coord_set, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.7 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.7 % (1396 of 1415)	98.2 % (726 of 739)	99.1 % (546 of 551)	99.2 % (124 of 125)
Backbone	98.9 % (712 of 720)	98.0 % (239 of 244)	99.4 % (360 of 362)	99.1 % (113 of 114)
Sidechain	98.6 % (800 of 811)	98.4 % (487 of 495)	99.0 % (302 of 305)	100.0 % (11 of 11)
Aromatic	97.4 % (152 of 156)	97.4 % (76 of 78)	97.2 % (70 of 72)	100.0 % (6 of 6)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. DysF

IDPFTADAGH TEFTDEVYQN ESRYPGGDWK PAEDTYTDAN GDKAASPSEL TCPPGWEWED DAWSYDINRA VDEKGWEYGI TIPPDHKPKS WVAAEKMYHT HRRRRLVRKR KKDLTQTASS TAR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.52 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K2O, Strand ID: A Detail

Release date

2008-06-29

Citation

Solution structure of the inner DysF domain of myoferlin and implications for limb girdle muscular dystrophy type 2b
Patel, P., Harris, R., Geddes, S.M., Strehle, E., Watson, J.D., Bashir, R., Bushby, K., Driscoll, P.C., Keep, N.H.
J. Mol. Biol. (2008), 379, 981-990, PubMed 18495154 , DOI 10.1016/j.jmb.2008.04.046 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9NZM1 released on 2002-01-23
Title MYOF_HUMAN Entity Myoferlin

Related entities 1. myoferlin, : 1 : 2 : 15 entities Detail

Interaction partners 1. myoferlin, : 45 interactors Detail

More details for 45 interactors identified by 6 citations

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

3 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

4 3D 1H-15N NOESY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

5 3D 1H-15N TOCSY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

6 3D HNCA

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

7 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

8 3D HNCACB

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

9 3D CBCA(CO)NH

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

10 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

11 3D HN(CA)CO

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

12 3D HA(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

13 3D HA(CACO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C-labelled DysF domain in 20 mM MES, 100 mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
6	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	D2O		10 %
10	H2O	natural abundance	90 %

14 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

15 aro-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

16 aro-TOCSY-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

17 aro-NOESY-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

18 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

19 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N/13C DysF in 20 mM MES, 100 mM NaCl, pH 6.5, in ~100% D2O

#	Name	Isotope labeling	Concentration
11	DysF	[U-100% 13C; U-100% 15N]	1.3 mM
12	MES	natural abundance	20 mM
13	sodium chloride	natural abundance	100 mM
14	D2O		100 %

20 IPAP

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

21 IPAP

Instrument

Varian UnityPlus - 500 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labelled DysF domain in 20mM MES, 100mM NaCl, pH 6.5

#	Name	Isotope labeling	Concentration
1	DysF	[U-100% 15N]	1.3 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O		10 %
5	H2O	natural abundance	90 %

NMR combined restraints 9 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_15718_2k2o.nef
Input source #2: Coordindates	2k2o.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--920-----930-------940-------950-------960-------970-------980-------990------1000------1010------1
IDPFTADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHT
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
IDPFTADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHT
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

020------1030------1040
HRRRRLVRKRKKDLTQTASSTAR
|||||||||||||||||||||||
HRRRRLVRKRKKDLTQTASSTAR
-------110-------120---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	123	0	0	100.0

Content subtype: combined_15718_2k2o.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	Chemical_Shift_List	Warning	1405		100.0 (chain: A, length: 123)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	409 (1)	noe	79.7 (chain: A, length: 123)
2	Distance restraints	CNS/XPLOR_distance_constraints_5	OK	525 (1)	noe	65.9 (chain: A, length: 123)
3	Distance restraints	CNS/XPLOR_distance_constraints_7	OK	560 (1)	noe	97.6 (chain: A, length: 123)
4	Distance restraints	CNS/XPLOR_distance_constraints_9	OK	169 (1)	noe	52.8 (chain: A, length: 123)
5	Distance restraints	CNS/XPLOR_distance_constraints_11	Warning	48 (1)	hbond	22.8 (chain: A, length: 123)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_10	OK	109 (109)	.	68.3 (chain: A, length: 123)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_12	OK	87 (87)	.	70.7 (chain: A, length: 123)

Assigned chemical shifts

Saveframe: Chemical_Shift_List
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 729
  - ¹³C chemical shifts: 544
  - ¹⁵N chemical shifts: 132
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	739	729	98.6
¹³C chemical shifts	551	544	98.7
¹⁵N chemical shifts	134	132	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	244	243	99.6
¹³C chemical shifts	246	244	99.2
¹⁵N chemical shifts	114	113	99.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	495	486	98.2
¹³C chemical shifts	305	300	98.4
¹⁵N chemical shifts	20	19	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	46	100.0
¹³C chemical shifts	46	46	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	78	76	97.4
¹³C chemical shifts	72	70	97.2
¹⁵N chemical shifts	6	6	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 79.7%
- Total number of restraints: 409
- Weight of restraints: 1.0 (409)
- Potential type of restraints: square-well-parabolic (409)
- Range of distance target values: 2.4, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_5
  - Status: OK
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 65.9%
  - Total number of restraints: 525
  - Weight of restraints: 1.0 (525)
  - Potential type of restraints: square-well-parabolic (525)
  - Range of distance target values: 2.4, 3.9 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_7
    - Status: OK
    - Experiment type: noe
    - Sequence coverage of experimental data
      - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 97.6%
    - Total number of restraints: 560
    - Weight of restraints: 1.0 (560)
    - Potential type of restraints: square-well-parabolic (560)
    - Range of distance target values: 2.4, 3.9 (Å)
    - Histogram of distance target values
    - Histogram of discrepancy in distance target values
    - Distance restraints per residue
      - Chain_ID: A
    - Distance restraints on contact map
    - Saveframe: CNS/XPLOR_distance_constraints_9
      - Status: OK
      - Experiment type: noe
      - Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 52.8%
        --920-----930-------940-------950-------960-------970-------980-------990------1000------1010------1 IDPFTADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHT || | | ||| || |||||||||||| |||||| ||| ||| | | | | |||||| | | |||||| |||||| ......DA.H.E..........RYP.GD...AEDTYTDANGDK.ASPSEL.CPP.WEW.D.A....I.R.VDEKGW......P.D.KPKSWV.AEKMYH. --920-----930-------940-------950-------960-------970-------980-------990------1000------1010------1 020------1030------1040 HRRRRLVRKRKKDLTQTASSTAR | | |||||| H.......K.KKDLTQ 020------1030---
      - Total number of restraints: 169
        Medium range restraints, 1 < | i - j | < 5 : 163
        Backbone-backbone: 38
        Backbone-side chain: 74
        Side chain-side chain: 51
        Long range restraints, | i - j | >= 5 : 6
      - Weight of restraints: 1.0 (169)
        Medium range restraints, 1 < | i - j | < 5 : 1.0 (163)
        Backbone-backbone: 1.0 (38)
        Backbone-side chain: 1.0 (74)
        Side chain-side chain: 1.0 (51)
        Long range restraints, | i - j | >= 5 : 1.0 (6)
      - Potential type of restraints: square-well-parabolic (169)
        Medium range restraints, 1 < | i - j | < 5 : square-well-parabolic (163)
        Backbone-backbone: square-well-parabolic (38)
        Backbone-side chain: square-well-parabolic (74)
        Side chain-side chain: square-well-parabolic (51)
        Long range restraints, | i - j | >= 5 : square-well-parabolic (6)
      - Range of distance target values: 3.4, 3.9 (Å)
      - Histogram of distance target values
      - Distance restraints per residue
        Chain_ID: A
      - Distance restraints on contact map
        Chain_ID_1: A - Chain_ID_2: A
      - Saveframe: CNS/XPLOR_distance_constraints_11
        Status: Warning
        Experiment type: hbond
        Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 22.8%
        Total number of restraints: 48
        Total hydrogen bond restraints: 48
        Weight of restraints: 1.0 (48)
        Total hydrogen bond restraints: 1.0 (48)
        Potential type of restraints: square-well-parabolic (48)
        Total hydrogen bond restraints: square-well-parabolic (48)
        Range of distance target values: 2.3, 3.3 (Å)
        Histogram of distance target values
        Distance restraints per residue
        Chain_ID: A
        Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_10
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 68.3%
- Total number of restraints: 109
- Total number of restraints per polymer type: 109
- Weight of restraints: 1.0 (109)
- Potential type of restraints: square-well-parabolic (109)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_12

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 70.7%

--920-----930-------940-------950-------960-------970-------980-------990------1000------1010------1
IDPFTADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHT
         ||||||||||||||| ||| | ||| ||||||| | | ||||   |||||||||||||||| |||||| ||||   || ||||||||||||
.........HTEFTDEVYQNESRY.GGD.K.AED.YTDANGD.A.S.SELT...GWEWEDDAWSYDINRA.DEKGWE.GITI...HK.KSWVAAEKMYHT
--920-----930-------940-------950-------960-------970-------980-------990------1000------1010------1

020------1030------1040
HRRRRLVRKRKKDLTQTASSTAR
||||| |||||||          
HRRRR.VRKRKKD          
020------1030

Total number of restraints: 87
Potential type of restraints: undefined (87)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords DysF, Dysferlin, Limb-girdle, Muscular Dystrophy, Myoferlin

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BMRB: 15718

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Chemical shift reference

Chemical shift reference

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Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. myoferlin, : 1 : 2 : 15 entities Detail

Interaction partners 1. myoferlin, : 45 interactors Detail

Experiments performed 21 experiments Detail

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NMR combined restraints 9 contents Detail