BMRB: 15744

Backbone assignment of lipid-free human apolipoprotein E (apoE)

Authors

Zhang, Y., Chen, J., Wang, J.

Assembly

Lipid-free human apolipoprotein E

Entity

1. Lipid-free human apolipoprotein E (polymer, Thiol state: all free), 308 monomers, 35375.33 Da Detail

MHHHHHHHHK VEQAVETEPE PELRQQTEWQ SGQRWELALG RFWDYLRWVQ TLSEQVQEEL LSSQVTQELR ALMDETMKEL KAYKSELEEQ LTPVAEETRA RLSKELQAAQ ARLGADMEDV CGRLVQYRGE VQAMLGQSTE ELRVRLASHL RKLRKRLLRD ADDLQKRLAV YQAGAREGAE RGLSAIRERL GPLVEQGRVR AATVGSLAGQ PLQERAQAWG ERLRARMEEM GSRTRDRLDE VKEQVAEVRA KLEEQAQQIR LQAEAAQARL KSRFEPLAED MQRQWAGQVE KVQAAEGTSA APVPSDNH

Formula weight

35375.33 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 38.1 %, Completeness (bb): 67.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	38.1 % (1373 of 3599)	22.5 % (428 of 1901)	48.2 % (655 of 1359)	85.5 % (290 of 339)
Backbone	67.0 % (1228 of 1832)	51.2 % (320 of 625)	68.2 % (619 of 907)	96.3 % (289 of 300)
Sidechain	20.7 % (426 of 2058)	8.5 % (108 of 1276)	42.7 % (317 of 743)	2.6 % (1 of 39)
Aromatic	0.0 % (0 of 164)	0.0 % (0 of 82)	0.0 % (0 of 76)	0.0 % (0 of 6)
Methyl	23.8 % (78 of 328)	14.6 % (24 of 164)	32.9 % (54 of 164)

1. Apolipoprotein E

MHHHHHHHHK VEQAVETEPE PELRQQTEWQ SGQRWELALG RFWDYLRWVQ TLSEQVQEEL LSSQVTQELR ALMDETMKEL KAYKSELEEQ LTPVAEETRA RLSKELQAAQ ARLGADMEDV CGRLVQYRGE VQAMLGQSTE ELRVRLASHL RKLRKRLLRD ADDLQKRLAV YQAGAREGAE RGLSAIRERL GPLVEQGRVR AATVGSLAGQ PLQERAQAWG ERLRARMEEM GSRTRDRLDE VKEQVAEVRA KLEEQAQQIR LQAEAAQARL KSRFEPLAED MQRQWAGQVE KVQAAEGTSA APVPSDNH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	Apolipoprotein E	[U-100% 13C; U-100% 15N; 80% 2H]		1 mM
2	phosphate buffer			25 mM
3	NaCl			25 mM
4	NaN3			0.05 mM
5	D2O			5 %
6	DTT-d10			10 mM
7	EDTA			10 mM

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LACS Plot; CA

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L7B, Strand ID: A Detail

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Release date

2008-10-14

Citation

A complete backbone spectral assignment of lipid-free human apolipoprotein E (apoE)
Zhang, Y., Chen, J., Wang, J.
Biomol. NMR Assign. (2008), 2, 207-210, PubMed 19636906 , DOI 10.1007/s12104-008-9122-8 , Abstract

Related entities 1. Lipid-free human apolipoprotein E, : 1 : 2 : 54 entities Detail

Interaction partners 1. Lipid-free human apolipoprotein E, : 39 interactors Detail

Experiments performed 4 experiments Detail