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BMRB: 15763

2K3J

The solution structure of human Mia40

Authors

Ciofi Baffoni, S., Bertini, I., Gallo, A.

Assembly

Human Mia40

Entity

1. Human Mia40 (polymer, Thiol state: free and disulfide bound), 146 monomers, 16388.62 Da Detail

GSFTMSYCRQ EGKDRIIFVT KEDHETPSSA ELVADDPNDP YEEHGLILPN GNINWNCPCL GGMASGPCGE QFKSAFSCFH YSTEEIKGSD CVDQFRAMQE CMQKYPDLYP QEDEDEEEER EKKPAEQAEE TAPIEATATK EEEGSS

Formula weight

16388.62 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS68:SG	1:CYS101:SG
2	disulfide	sing	1:CYS78:SG	1:CYS91:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 56.2 %, Completeness: 48.4 %, Completeness (bb): 53.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.4 % (791 of 1633)	48.6 % (417 of 858)	47.0 % (294 of 626)	53.7 % (80 of 149)
Backbone	53.0 % (454 of 856)	53.8 % (157 of 292)	52.6 % (225 of 428)	52.9 % (72 of 136)
Sidechain	45.0 % (411 of 913)	45.9 % (260 of 566)	42.8 % (143 of 334)	61.5 % (8 of 13)
Aromatic	17.7 % (22 of 124)	35.5 % (22 of 62)	0.0 % (0 of 61)	0.0 % (0 of 1)
Methyl	52.2 % (47 of 90)	51.1 % (23 of 45)	53.3 % (24 of 45)

1. Human Mia40

GSFTMSYCRQ EGKDRIIFVT KEDHETPSSA ELVADDPNDP YEEHGLILPN GNINWNCPCL GGMASGPCGE QFKSAFSCFH YSTEEIKGSD CVDQFRAMQE CMQKYPDLYP QEDEDEEEER EKKPAEQAEE TAPIEATATK EEEGSS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Human Mia40	[U-100% 15N]	0.5-1 mM
2	DTT	natural abundance	2 mM
3	potassium phosphate	natural abundance	50 mM
4	EDTA	natural abundance	0.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	Human Mia40	[U-100% 13C; U-100% 15N]	0.5-1 mM
6	DTT	natural abundance	2 mM
7	potassium phosphate	natural abundance	50 mM
8	EDTA	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.21 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.21 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.96 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K3J, Strand ID: A Detail

Release date

2009-02-08

Citation

MIA40 is an oxidoreductase that catalyzes oxidative protein folding in mitochondria
Cefaro, C., Banci, L., Bertini, I., Ciofi-Baffoni, S., Gallo, A., Martinelli, M., Dionisia, S.P., Nitsa, K., Tokatlidis, K.
Nat. Struct. Mol. Biol. (2009), 16, 198-206, PubMed 19182799 , DOI 10.1038/nsmb.1553 , Abstract

Related entities 1. Human Mia40, : 1 : 3 : 9 entities Detail

Interaction partners 1. Human Mia40, : 8 interactors Detail

More details for 8 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC