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BMRB: 15774

2K3M

Rv1761c

Authors

Page, R.C., Moore, J.D., Lee, S., Opella, S.J., Cross, T.A.

Assembly

Rv1761c

Entity

1. Rv1761c (polymer, Thiol state: not present), 151 monomers, 16225.21 Da Detail

MHHHHHHSSG VDLGTENLYF QSNAMSDFDT ERVSRAVAAA LVGPGGVALV VKVFAGLPGV IHTPARRGFF RSNPERIQIG DWRYEVAHDG RLLAAHMVNG IVIAEDALIA EAVGPHLARA LGQIVSRYGA TVIPNINAAI EVLGTGTDYR F

2. MTN (non-polymer), 264.385 Da

Total weight

16489.596 Da

Max. entity weight

16225.21 Da

Source organism

Mycobacterium tuberculosis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 86.8 %, Completeness: 39.0 %, Completeness (bb): 67.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.0 % (648 of 1662)	20.4 % (172 of 845)	53.6 % (355 of 662)	78.1 % (121 of 155)
Backbone	67.0 % (599 of 894)	43.9 % (137 of 312)	78.0 % (341 of 437)	83.4 % (121 of 145)
Sidechain	16.8 % (152 of 903)	6.6 % (35 of 533)	32.5 % (117 of 360)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 144)	0.0 % (0 of 72)	0.0 % (0 of 71)	0.0 % (0 of 1)
Methyl	18.5 % (37 of 200)	10.0 % (10 of 100)	27.0 % (27 of 100)

1. Rv1761c

MHHHHHHSSG VDLGTENLYF QSNAMSDFDT ERVSRAVAAA LVGPGGVALV VKVFAGLPGV IHTPARRGFF RSNPERIQIG DWRYEVAHDG RLLAAHMVNG IVIAEDALIA EAVGPHLARA LGQIVSRYGA TVIPNINAAI EVLGTGTDYR F

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details F30C-MTSL labeled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
1	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
2	CYSP	natural abundance	0.4 (±0.05) mM
3	D2O	natural abundance	10 (±1.0) %
4	H2O	natural abundance	90 (±1.0) %
5	sodium acetate	natural abundance	20 (±1.0) mM
6	DPC	natural abundance	150 (±5.0) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details S48C-MTSL labled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
7	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
8	CYSP	natural abundance	0.4 (±0.05) mM
9	D2O	natural abundance	10 (±1.0) %
10	H2O	natural abundance	90 (±1.0) %
11	sodium acetate	natural abundance	20 (±1.0) mM
12	DPC	natural abundance	150 (±5.0) mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details S102C-MTSL labeled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
13	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
14	CYSP	natural abundance	0.4 (±0.05) mM
15	D2O	natural abundance	10 (±1.0) %
16	H2O	natural abundance	90 (±1.0) %
17	sodium acetate	natural abundance	20 (±1.0) mM
18	DPC	natural abundance	150 (±5.0) mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
24	Rv1761c	[U-100% 15N]	1 (±0.05) mM
25	D2O	natural abundance	10 (±1.0) %
26	H2O	natural abundance	90 (±1.0) %
27	sodium acetate	natural abundance	20 (±1.0) mM
28	DPC	natural abundance	150 (±5.0) mM

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details neutral gel sample for RDC

#	Name	Isotope labeling	Concentration
29	Rv1761c	[U-100% 15N]	1 (±0.05) mM
30	D2O	natural abundance	10 (±1.0) %
31	H2O	natural abundance	90 (±1.0) %
32	sodium acetate	natural abundance	20 (±1.0) mM
33	DPC	natural abundance	150 (±5.0) mM
34	polyacrylamide gel	natural abundance	5 (±0.1) %

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details negatively charged gel sample for RDC

#	Name	Isotope labeling	Concentration
35	Rv1761c	[U-100% 15N]	1 (±0.05) mM
36	D2O	natural abundance	10 (±1.0) %
37	H2O	natural abundance	90 (±1.0) %
38	sodium acetate	natural abundance	20 (±1.0) mM
39	DPC	natural abundance	150 (±5.0) mM
40	polyacrylamide gel	natural abundance	4.4 (±0.1) %
41	2-acrylamido-2-methyl-1-propanesulfonic acid	natural abundance	1.1 (±0.1) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.61 ppm, Outliers: 8 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 2K3M, Strand ID: A Detail

RDC

217 RDC values in 2 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02) Detail

Release date

2008-12-29

Citation

Backbone structure of a small helical integral membrane protein: A unique structural characterization
Page, R.C., Lee, S., Moore, J.D., Opella, S.J., Cross, T.A.
Protein Sci. (2009), 18, 134-146, PubMed 19177358 , DOI 10.1002/pro.24 , Abstract

Related entities 1. Rv1761c, : 1 : 1 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

2 2D 1H-15N HSQC

Instrument

Varian Avance - 900 MHz ultra-wide bore 900MHz at National High Magnetic Field Laboratory

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details F30C-MTSL labeled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
1	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
2	CYSP	natural abundance	0.4 (±0.05) mM
3	D2O	natural abundance	10 (±1.0) %
4	H2O	natural abundance	90 (±1.0) %
5	sodium acetate	natural abundance	20 (±1.0) mM
6	DPC	natural abundance	150 (±5.0) mM

3 2D 1H-15N HSQC

Instrument

Varian Avance - 900 MHz ultra-wide bore 900MHz at National High Magnetic Field Laboratory

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details S48C-MTSL labled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
7	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
8	CYSP	natural abundance	0.4 (±0.05) mM
9	D2O	natural abundance	10 (±1.0) %
10	H2O	natural abundance	90 (±1.0) %
11	sodium acetate	natural abundance	20 (±1.0) mM
12	DPC	natural abundance	150 (±5.0) mM

4 2D 1H-15N HSQC

Instrument

Varian Avance - 900 MHz ultra-wide bore 900MHz at National High Magnetic Field Laboratory

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details S102C-MTSL labeled Rv1761c sample used to collect PRE data. cysteine residue with covalently attached MTSL spin label [(1-Oxyl-2,2,5,5-tetramethyl-d-3-pyrroline-3-methyl) methanethiosulfonate].

#	Name	Isotope labeling	Concentration
13	Rv1761c	[U-100% 15N]	0.4 (±0.05) mM
14	CYSP	natural abundance	0.4 (±0.05) mM
15	D2O	natural abundance	10 (±1.0) %
16	H2O	natural abundance	90 (±1.0) %
17	sodium acetate	natural abundance	20 (±1.0) mM
18	DPC	natural abundance	150 (±5.0) mM

5 3D HNCO

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

6 3D HNCA

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

8 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
19	Rv1761c	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
20	D2O	natural abundance	10 (±1.0) %
21	H2O	natural abundance	90 (±1.0) %
22	sodium acetate	natural abundance	20 (±1.0) mM
23	DPC	natural abundance	150 (±5.0) mM

10 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
24	Rv1761c	[U-100% 15N]	1 (±0.05) mM
25	D2O	natural abundance	10 (±1.0) %
26	H2O	natural abundance	90 (±1.0) %
27	sodium acetate	natural abundance	20 (±1.0) mM
28	DPC	natural abundance	150 (±5.0) mM

11 2D 1H-15N HSQC

Instrument

Varian INOVA - 720 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
24	Rv1761c	[U-100% 15N]	1 (±0.05) mM
25	D2O	natural abundance	10 (±1.0) %
26	H2O	natural abundance	90 (±1.0) %
27	sodium acetate	natural abundance	20 (±1.0) mM
28	DPC	natural abundance	150 (±5.0) mM

12 3D 1H-15N NOESY

Instrument

Varian INOVA - 720 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details sample used for assignments

#	Name	Isotope labeling	Concentration
24	Rv1761c	[U-100% 15N]	1 (±0.05) mM
25	D2O	natural abundance	10 (±1.0) %
26	H2O	natural abundance	90 (±1.0) %
27	sodium acetate	natural abundance	20 (±1.0) mM
28	DPC	natural abundance	150 (±5.0) mM

13 2D 1H-15N HSQC-IPAP

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details neutral gel sample for RDC

#	Name	Isotope labeling	Concentration
29	Rv1761c	[U-100% 15N]	1 (±0.05) mM
30	D2O	natural abundance	10 (±1.0) %
31	H2O	natural abundance	90 (±1.0) %
32	sodium acetate	natural abundance	20 (±1.0) mM
33	DPC	natural abundance	150 (±5.0) mM
34	polyacrylamide gel	natural abundance	5 (±0.1) %

14 2D 1H-15N HSQC-IPAP

Instrument

Bruker Avance - 600 MHz cryoprobe equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 (±0.1) K, pH 4 (±0.02), Details negatively charged gel sample for RDC

#	Name	Isotope labeling	Concentration
35	Rv1761c	[U-100% 15N]	1 (±0.05) mM
36	D2O	natural abundance	10 (±1.0) %
37	H2O	natural abundance	90 (±1.0) %
38	sodium acetate	natural abundance	20 (±1.0) mM
39	DPC	natural abundance	150 (±5.0) mM
40	polyacrylamide gel	natural abundance	4.4 (±0.1) %
41	2-acrylamido-2-methyl-1-propanesulfonic acid	natural abundance	1.1 (±0.1) %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Dihedral angle restraints, RDC restraints	combined_15774_2k3m.nef
Input source #2: Coordindates	2k3m.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:54:CYS:SG	2:1:MTN:S1	unknown	unknown	n/a
1:72:CYS:SG	2:2:MTN:S1	unknown	unknown	n/a
1:126:CYS:SG	2:3:MTN:S1	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
B	1	MTN	S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate	None
B	2	MTN	S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate	None
B	3	MTN	S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------------------------------10--------20--------30--------40--------50--------60--------70------
MHHHHHHSSGVDLGTENLYFQSNAMSDFDTERVSRAVAAALVGPGGVALVVKVCAGLPGVIHTPARRGFFRCNPERIQIGDWRYEVAHDGRLLAAHMVNG
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MHHHHHHSSGVDLGTENLYFQSNAMSDFDTERVSRAVAAALVGPGGVALVVKVCAGLPGVIHTPARRGFFRCNPERIQIGDWRYEVAHDGRLLAAHMVNG
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--80--------90-------100-------110-------120-------
IVIAEDALIAEAVGPHLARALGQIVCRYGATVIPNINAAIEVLGTGTDYRF
|||||||||||||||||||||||||||||||||||||||||||||||||||
IVIAEDALIAEAVGPHLARALGQIVCRYGATVIPNINAAIEVLGTGTDYRF
-------110-------120-------130-------140-------150-

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	151	0	0	100.0

Content subtype: combined_15774_2k3m.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	3		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Error	533		No information
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	169 (169)	.	66.9 (chain: A, length: 151)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_2	OK	166 (166)	.	58.9 (chain: A, length: 151)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Error

Covalent bonds

Saveframe: assembly
- Status: OK

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 151, Sequence coverage: 66.9%
- Total number of restraints: 169
- Total number of restraints per polymer type: 169
- Weight of restraints: 1.0 (169)
- Potential type of restraints: square-well-parabolic (169)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_2

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 151, Sequence coverage: 58.9%

--------------------------------10--------20--------30--------40--------50--------60--------70------
MHHHHHHSSGVDLGTENLYFQSNAMSDFDTERVSRAVAAALVGPGGVALVVKVCAGLPGVIHTPARRGFFRCNPERIQIGDWRYEVAHDGRLLAAHMVNG
 |||||||| ||||| ||||||||| | ||||||| ||||||| |||| |||||||| ||||| || ||||||  |||||||||| ||||||||||||||
.HHHHHHSS.VDLGT.NLYFQSNAM.D.DTERVSR.VAAALVG.GGVA.VVKVCAGL.GVIHT.AR.GFFRCN..RIQIGDWRYE.AHDGRLLAAHMVNG
--------------------------------10--------20--------30--------40--------50--------60--------70------

--80--------90-------100-------110-------120-------
IVIAEDALIAEAVGPHLARALGQIVCRYGATVIPNINAAIEVLGTGTDYRF
|||                                                
IVI                                                
---

Total number of restraints: 166
Potential type of restraints: undefined (166)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords detergent micelle, integral membrane protein, Mycobacterium tuberculosis, paramagnetic relaxation enhancement, residual dipolar couplings, solution NMR, PROTEIN

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Found 1 Document (0.92 seconds)

BMRB: 15774

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Sample #7

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Rv1761c, : 1 : 1 entities Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 4 contents Detail