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BMRB: 15776

2K3R

Pfu Rpp21 structure and assignments

Authors

Amero, C.D., Foster, M.P., Boomershine, W.P., Xu, Y.

Assembly

Rpp21-Zn

Entity

1. Rpp21 (polymer, Thiol state: all other bound), 123 monomers, 14882.90 Da Detail

MAKYNEKKEK KRIAKERIDI LFSLAERVFP YSPELAKRYV ELALLVQQKA KVKIPRKWKR RYCKKCHAFL VPGINARVRL RQKRMPHIVV KCLECGHIMR YPYIKEIKKR RKEKMEYGGL VPR

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

14948.31 Da

Max. entity weight

14882.9 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS63:SG	2:ZN1:ZN
2	na	sing	1:CYS66:SG	2:ZN1:ZN
3	na	sing	1:CYS92:SG	2:ZN1:ZN
4	na	sing	1:CYS95:SG	2:ZN1:ZN

Source organism

Pyrococcus furiosus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 71.5 %, Completeness: 59.6 %, Completeness (bb): 65.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.6 % (963 of 1615)	55.8 % (486 of 871)	63.5 % (395 of 622)	67.2 % (82 of 122)
Backbone	65.7 % (476 of 724)	66.7 % (162 of 243)	64.7 % (236 of 365)	67.2 % (78 of 116)
Sidechain	56.2 % (568 of 1010)	51.6 % (324 of 628)	63.8 % (240 of 376)	66.7 % (4 of 6)
Aromatic	63.6 % (70 of 110)	63.6 % (35 of 55)	63.0 % (34 of 54)	100.0 % (1 of 1)
Methyl	82.8 % (106 of 128)	82.8 % (53 of 64)	82.8 % (53 of 64)

1. Rpp21

MAKYNEKKEK KRIAKERIDI LFSLAERVFP YSPELAKRYV ELALLVQQKA KVKIPRKWKR RYCKKCHAFL VPGINARVRL RQKRMPHIVV KCLECGHIMR YPYIKEIKKR RKEKMEYGGL VPR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
16	TRIS	[U-99% 2H]	10 mM
17	potassium chloride	natural abundance	10 mM
18	sodium azide	natural abundance	0.02 %
19	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
20	ZINC chloride	natural abundance	0.3 mM
21	H2O	natural abundance	90 %
22	D2O		10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.52 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.52 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K3R, Strand ID: A Detail

Release date

2008-11-13

Citation

Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner
Amero, C.D., Boomershine, W.P., Xu, Y., Foster, M.
Biochemistry (2008), 47, 11704-11710, PubMed 18922021 , DOI 10.1021/bi8015982 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q8U0H6 released on 2004-07-05
    Title RNP4_PYRFU Entity Ribonuclease P protein component 4
  Swiss-Prot: Q8U007 released on 2004-03-29
    Title RNP1_PYRFU Entity Ribonuclease P protein component 1

Related entities 1. Rpp21, : 1 : 1 : 1 : 19 entities Detail

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

2 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

3 3D HNCA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

4 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

5 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

6 3D 1H-15N TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

7 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

8 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

9 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
16	TRIS	[U-99% 2H]	10 mM
17	potassium chloride	natural abundance	10 mM
18	sodium azide	natural abundance	0.02 %
19	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
20	ZINC chloride	natural abundance	0.3 mM
21	H2O	natural abundance	90 %
22	D2O		10 %

10 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

11 3D HCCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

12 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Rpp21

#	Name	Isotope labeling	Concentration
1	TRIS	[U-99% 2H]	10 mM
2	potassium chloride	natural abundance	10 mM
3	sodium azide	natural abundance	0.02 %
4	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
5	ZINC chloride	natural abundance	0.3 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

13 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

14 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

15 3D HNCA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

16 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

17 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details Pfu Rpp21-Rpp29

#	Name	Isotope labeling	Concentration
8	TRIS	[U-99% 2H]	10 mM
9	potassium chloride	natural abundance	10 mM
10	sodium azide	natural abundance	0.02 %
11	Rpp21	[U-100% 13C; U-100% 15N]	0.5 mM
12	ZINC chloride	natural abundance	0.3 mM
13	Rpp29	natural abundance	0.5 mM
14	H2O	natural abundance	90 %
15	D2O		10 %

18 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

19 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

20 3D HNCA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

21 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

22 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 6.7, Details cobalt-substituted Rpp21

#	Name	Isotope labeling	Concentration
23	TRIS	[U-99% 2H]	10 mM
24	potassium chloride	natural abundance	10 mM
25	sodium azide	natural abundance	0.02 %
26	Rpp21	[U-100% 13C; U-100% 15N]	1 mM
27	cobalt chloride	natural abundance	0.3 mM
28	H2O	natural abundance	90 %
29	D2O		10 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_15776_2k3r.nef
Input source #2: Coordindates	2k3r.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:63:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:66:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:92:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:95:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
B	1	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MAKYNEKKEKKRIAKERIDILFSLAERVFPYSPELAKRYVELALLVQQKAKVKIPRKWKRRYCKKCHAFLVPGINARVRLRQKRMPHIVVKCLECGHIMR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAKYNEKKEKKRIAKERIDILFSLAERVFPYSPELAKRYVELALLVQQKAKVKIPRKWKRRYCKKCHAFLVPGINARVRLRQKRMPHIVVKCLECGHIMR

-------110-------120---
YPYIKEIKKRRKEKMEYGGLVPR
|||||||||||||||||||||||
YPYIKEIKKRRKEKMEYGGLVPR

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	123	0	0	100.0

Content subtype: combined_15776_2k3r.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	4		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	901		65.0 (chain: A, length: 123)
1	Distance restraints	CNS/XPLOR_distance_constraints_5	Warning	1580 (1)	noe	65.0 (chain: A, length: 123)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	68 (1)	hbond	35.0 (chain: A, length: 123)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 65.0%
- Total number of assignments
  - ¹H chemical shifts: 456
  - ¹³C chemical shifts: 370
  - ¹⁵N chemical shifts: 75
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	871	456	52.4
¹³C chemical shifts	622	370	59.5
¹⁵N chemical shifts	137	75	54.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	243	153	63.0
¹³C chemical shifts	246	142	57.7
¹⁵N chemical shifts	116	71	61.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	628	303	48.2
¹³C chemical shifts	376	228	60.6
¹⁵N chemical shifts	21	4	19.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	51	75.0
¹³C chemical shifts	68	50	73.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	35	63.6
¹³C chemical shifts	54	34	63.0
¹⁵N chemical shifts	1	1	100.0

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_5
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 65.0%
- Total number of restraints: 1574
- Weight of restraints: 1.0 (1574)
- Potential type of restraints: square-well-parabolic (1574)
- Range of distance target values: 2.65, 4.93 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
    None
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 35.0%
  - Total number of restraints: 68
  - Weight of restraints: 1.0 (68)
  - Potential type of restraints: square-well-parabolic (68)
  - Range of distance target values: 1.9, 2.85 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
      None
  - Distance restraints on contact map

Keywords NMR, RNase P, Rpp21, Pfu Rpp21

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Found 1 Document (0.847 seconds)

BMRB: 15776

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. Rpp21, : 1 : 1 : 1 : 19 entities Detail

Experiments performed 22 experiments Detail

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