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BMRB: 15790

2K48

NMR STRUCTURE OF THE N-TERMINAL COILED COIL DOMAIN OF THE ANDES HANTAVIRUS NUCLEOCAPSID PROTEIN

Authors

Wang, Y., Boudreaux, D.M., Estrada, D.F., Egan, C.W., St. Jeor, S.C., De Guzman, R.N.

Assembly

ANDES HANTAVIRUS NUCLEOCAPSID PROTEIN

Entity

1. ANDES HANTAVIRUS NUCLEOCAPSID PROTEIN (polymer, Thiol state: not present), 107 monomers, 12052.46 Da Detail

MHHHHHHGKP IPNPLLGLDS TENLYFQGID PFTMSTLQEL QENITAHEQQ LVTARQKLKD AEKAVEVDPD DVNKSTLQNR RAAVSTLETK LGELKRQLAD LVAAQKL

Formula weight

12052.46 Da

Source organism

Andes orthohantavirus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 85.7 %, Completeness (bb): 93.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (1073 of 1252)	84.9 % (555 of 654)	87.3 % (421 of 482)	83.6 % (97 of 116)
Backbone	93.4 % (590 of 632)	94.4 % (201 of 213)	92.4 % (293 of 317)	94.1 % (96 of 102)
Sidechain	80.2 % (580 of 723)	80.3 % (354 of 441)	84.0 % (225 of 268)	7.1 % (1 of 14)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 28)
Methyl	93.8 % (122 of 130)	93.8 % (61 of 65)	93.8 % (61 of 65)

1. ANDES HANTAVIRUS NUCLEOCAPSID PROTEIN

MHHHHHHGKP IPNPLLGLDS TENLYFQGID PFTMSTLQEL QENITAHEQQ LVTARQKLKD AEKAVEVDPD DVNKSTLQNR RAAVSTLETK LGELKRQLAD LVAAQKL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.0 (±0.1) mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	10 mM
4	H2O	natural abundance	90 %
5	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
6	entity	[U-100% 13C; U-100% 15N]	1.0 (±0.1) mM
7	sodium phosphate	natural abundance	10 mM
8	sodium chloride	natural abundance	10 mM
9	D2O		100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
10	entity	[U-100% 15N]	1.0 (±0.1) mM
11	sodium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	10 mM
13	H2O	natural abundance	90 %
14	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K48, Strand ID: A Detail

Release date

2008-07-28

Citation

NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein
Wang, Y., Boudreaux, D.M., Estrada, D.F., Egan, C.W., St. Jeor, S.C., De Guzman, R.N.
J. Biol. Chem. (2008), 283, 28297-28304, PubMed 18687679 , DOI 10.1074/jbc.M804869200 , Abstract

Related entities 1. ANDES HANTAVIRUS NUCLEOCAPSID PROTEIN, : 1 : 2 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC