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NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178
Authors
SINGARAPU, K., WU, Y., HUA, J., SUKUMARAN, D., Zhao, L., Jiang, M., Foote, E.L., Xiao, R., Nair, R., Baran, M.C., G.V.T, S., Acton, T., Rost, B., Montelione, G.T., SZYPERSKI, T.
Assembly
FeoA-like protein
Entity
1. FeoA-like protein (polymer, Thiol state: all free), 86 monomers, 9703.386 Da Detail

MTKGIGLNEV EIKSKVKVIG IVPESKVRRK IMDMGIVRGT EIYIEGKAPM GDPIALRLRG YSLSLRKSEA KDILVEVLLE HHHHHH


Formula weight
9703.386 Da
Source organism
Clostridium acetobutylicum
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.3 %, Completeness: 91.5 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.5 % (938 of 1025)91.8 % (494 of 538)90.8 % (366 of 403)92.9 % (78 of 84)
Backbone92.4 % (471 of 510)93.8 % (166 of 177)91.2 % (228 of 250)92.8 % (77 of 83)
Sidechain90.9 % (539 of 593)90.9 % (328 of 361)90.9 % (210 of 231)100.0 % (1 of 1)
Aromatic50.0 % (20 of 40)60.0 % (12 of 20)40.0 % (8 of 20)
Methyl98.2 % (112 of 114)96.5 % (55 of 57)100.0 % (57 of 57)

1. FeoA-like protein

MTKGIGLNEV EIKSKVKVIG IVPESKVRRK IMDMGIVRGT EIYIEGKAPM GDPIALRLRG YSLSLRKSEA KDILVEVLLE HHHHHH

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1entity[U-100% 13C; U-100% 15N]0.91 mM

LACS Plot; CA
Referencing offset: -0.07 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.07 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.1 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.56 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2K4Y, Strand ID: A Detail


Release date
2008-08-07
Citation
NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178
SINGARAPU, K., WU, Y., HUA, J., SUKUMARAN, D., Zhao, L., Jiang, M., Foote, E.L., Xiao, R., Nair, R., Baran, M.C., G.V.T, S., Acton, T., Rost, B., Montelione, G.T., SZYPERSKI, T.
Not known
Related entities 1. FeoA-like protein, : 1 : 4 entities Detail
Experiments performed 7 experiments Detail
NMR combined restraints 4 contents Detail
Keywords GFT NMR, Iron binding protein, Structural Genomics