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BMRB: 15841

2K5L

Solution NMR Structure of Protein FeoA from Clostridium thermocellum, Northeast Structural Genomics Consortium Target CmR17

Authors

Zeri, A., Singarapu, K.K., Mills, J.L., Wu, Y., Garcia, E., Wang, H., Jiang, M., Foote, E.L., Xiao, R., Nair, R., Everett, J.K., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Assembly

Protein FeoA

Entity

1. Protein FeoA (polymer, Thiol state: all free), 81 monomers, 9227.730 Da Detail

MFSLRDAKCG QTVKVVKLHG TGALKRRIMD MGITRGCEIY IRKVAPLGDP IQINVRGYEL SLRKSAAEMI EVELEHHHHH H

Formula weight

9227.73 Da

Source organism

Acetivibrio thermocellus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (918 of 952)	95.6 % (476 of 498)	96.8 % (360 of 372)	100.0 % (82 of 82)
Backbone	97.3 % (469 of 482)	95.8 % (160 of 167)	97.5 % (230 of 236)	100.0 % (79 of 79)
Sidechain	96.0 % (522 of 544)	95.5 % (316 of 331)	96.7 % (203 of 210)	100.0 % (3 of 3)
Aromatic	81.5 % (44 of 54)	85.2 % (23 of 27)	77.8 % (21 of 27)
Methyl	99.0 % (95 of 96)	97.9 % (47 of 48)	100.0 % (48 of 48)

1. CmR17

MFSLRDAKCG QTVKVVKLHG TGALKRRIMD MGITRGCEIY IRKVAPLGDP IQINVRGYEL SLRKSAAEMI EVELEHHHHH H

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	90 %
3	D2O	[U-100% 2H]	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-10% 13C; U-100% 15N]	1.2 mM
5	H2O	natural abundance	90 %
6	D2O	[U-100% 2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K5L, Strand ID: A Detail

Release date

2008-08-18

Citation

Solution NMR Structure of Protein FeoA from Clostridium thermocellum, Northeast Structural Genomics Consortium Target CmR17
Zeri, A., Singarapu, K.K., Mills, J.L., Wu, Y., Garcia, E., Wang, H., Jiang, M., Foote, E.L., Xiao, R., Nair, R., Everett, J.K., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Not known

Related entities 1. Protein FeoA, : 1 : 13 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-13C HSQC